dc.contributor.author | Tajber, Lidia | |
dc.contributor.author | Sladek, Svenja | |
dc.contributor.author | Kearney, Clodagh | |
dc.contributor.author | Crean, Daniel | |
dc.contributor.author | Brama, Pieter A.J. | |
dc.contributor.author | Fawcett, Karolina | |
dc.contributor.author | Labberte, Margot C. | |
dc.contributor.author | Leggett, Bernadette | |
dc.contributor.author | Brayden, David J. | |
dc.date.accessioned | 2019-11-25T16:11:50Z | |
dc.date.available | 2019-11-25T16:11:50Z | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018 | en |
dc.identifier.citation | Sladek, S., Kearney, C., Crean, D., Brama, P.A.J., Tajber, L., Fawcett, K., Labberte, M.C., Leggett, B. & Brayden, D.J., Intra-articular delivery of a nanocomplex comprising salmon calcitonin, hyaluronic acid, and chitosan using an equine model of joint inflammation, Drug Delivery and Translational Research, 8, 5, 2018, 1421 - 1435 | en |
dc.identifier.other | Y | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs13346-018-0557-x | |
dc.identifier.uri | http://hdl.handle.net/2262/90877 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Polyelectrolyte nanoparticle constructs (NPs) comprising salmon calcitonin (sCT), chitosan (CS), and hyaluronic acid (HA) were previously established as having anti-inflammatory potential when injected via the intra-articular (i.a.) route to a mouse model. We attempted to translate the formulation to a large animal model, the lipopolysaccharide (LPS)-stimulated equine model of joint inflammation. The aim was to manufacture under aseptic conditions to produce sterile pyrogen-free NPs, to confirm physicochemical characteristics, and to test toxicity and efficacy in a pilot study. NP dispersions were successfully formulated using pharmaceutical-grade source materials and were aseptically manufactured under GMP-simulated conditions in a grade A modular aseptic processing workstation. The NP formulation had no detectable pathogen or endotoxin contamination. NPs were then tested versus a lactated Ringer’s solution control following single i.a. injections to the radiocarpal joints of two groups of four horses pre-treated with LPS, followed by arthrocentesis at set intervals over 1 week. There was no evidence of treatment-related toxicity over the period. While there were no differences between clinical read-outs of the NP and the control, two synovial fluid-derived biomarkers associated with cartilage turnover revealed a beneficial effect of NPs. In conclusion, NPs comprising well-known materials were manufactured for an equine i.a.-injectable pilot study and yielded no NP-attributable toxicity. Evidence of NP-associated benefit at the level of secondary endpoints was detected as a result of decreases in synovial fluid inflammatory biomarkers. | en |
dc.format.extent | 1421 | en |
dc.format.extent | 1435 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Drug Delivery and Translational Research; | |
dc.relation.ispartofseries | 8; | |
dc.relation.ispartofseries | 5; | |
dc.rights | Y | en |
dc.subject | Salmon calcitonin | en |
dc.subject | Hylauronic acid | en |
dc.subject | Chitosan | en |
dc.subject | Joint inflammation | en |
dc.subject | Synovitis | en |
dc.subject | Nanomedicine | en |
dc.subject | Large animal models | en |
dc.title | Intra-articular delivery of a nanocomplex comprising salmon calcitonin, hyaluronic acid, and chitosan using an equine model of joint inflammation | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/ltajber | |
dc.identifier.rssinternalid | 190438 | |
dc.identifier.doi | http://dx.doi.org/10.1007/s13346-018-0557-x | |
dc.rights.ecaccessrights | openAccess | |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.subject.TCDTheme | Nanoscience & Materials | en |
dc.subject.TCDTag | ANTI-INFLAMMATORY DRUGS | en |
dc.subject.TCDTag | CHITOSAN | en |
dc.subject.TCDTag | HYALURONATE | en |
dc.subject.TCDTag | polyelectrolyte nanoparticles | en |
dc.subject.TCDTag | salmon calcitonin | en |
dc.identifier.orcid_id | 0000-0003-1544-6796 | |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsorGrantNumber | 12/RC/2275 | en |