Browsing Genetics by Date of Publication
Now showing items 21-40 of 550
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Testing the hypothesis of genome duplications in vertebrates
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2000)The hypothesis that the human genome has undergone at least two rounds of genome duplication has become widely accepted, but has never been rigorously tested. In this study, several map-based inter- and intra-species ... -
Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2
(2001)Smac/DIABLO is a mitochondrial protein that potentiates some forms of apoptosis, possibly by neutralizing one or more members of the IAP family of apoptosis inhibitory proteins. Smac has been shown to exit mitochondria and ... -
CARDINAL, a novel caspase recruitment domain protein, is an inhibitor of multiple NF-kappa B activation pathways.
(2001)Proteins possessing the caspase recruitment domain (CARD) motif have been implicated in pathways leading to activation of caspases or NF-kappaB in the context of apoptosis or inflammation, respectively. Here we report the ... -
Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis.
(2001)Members of the caspase family of cysteine proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Because the major executioner caspases, caspase-3 and caspase-7, exhibit almost ... -
A leaf-specific phage T7 RNA polymerase-based system for transgene expression in tobacco chloroplasts
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2001)This thesis describes the development of a chloroplast-localised gene expression system based on the phage T7 RNA polymerase. In order to direct T7 RNAP expression in a leaf-specific manner and to target it to the ... -
Characterization of two genes transcribed from a divergent promotor in Arabidopsis thaliana and encoding plastid-targeted proteins
(Trinity College (Dublin, Ireland). Department of Genetics, 2001)As part of the Arabidopsis genome sequencing project, approximately 85kb of sequence data on chromosome 4 was generated in this laboratory. This included a cosmid clone called cAt4S5 containing a genomic insert of 13.3kb ... -
Therapeutic approaches to halting photoreceptor cell death in the retina
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2001)Retinitis pigmentosa (RP) is a group of hereditary retinopathies affecting approximately 1 in 3500 people in the developed world. The disorders are characterised by the early onset of night blindness followed by a ... -
Comparative analysis of human and mouse acute phase Serum Amyloid A gene expression
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2001)The Serum Amyloid A (S AA) proteins are a family of highly conserved lipoproteins secreted by the liver. Their production and release is massively induced under conditions of inflammation or infection by pro-inflammatory ... -
An investigation of the role of caspase-9-interacting proteins in the molecular control of apoptosis
(Trinity College (Dublin, Ireland). Department of Genetics, 2001)Apoptosis is an important homeostatic mechanism that multicellular organisms utilize to delete cells during development and on an ongoing basis in the adult. At the molecular level, this requires the activation of a family ... -
On the genetics of retinitis pigmentosa and on mutation-independent approaches to therapeutic intervention
(Nature Publishing Group, 2002)Retinitis pigmentosa (RP), the group of hereditary conditions involving death of retinal photoreceptors, represents the most prevalent cause of visual handicap among working populations in developed countries. Here we ... -
Transformation and marker gene removal strategies for the plastid genome of Brassica napus
(Trinity College (Dublin, Ireland). Department of Genetics, 2002)Despite the fact that the first transplastomic higher plant was produced more than a decade ago, transformation of the plastid genome and the regeneration of homoplasmic, fertile plants has so far been achieved in only 2 ... -
Genome sequencing and functional genomics in Bacillus subtilis : the expression and roles of the superoxide dismutases and NADH dehydrogenases
(Trinity College (Dublin, Ireland). Department of Genetics, 2002)The work in this thesis spans genome sequencing of Bacillus subtilis, functional analysis of genes of unknown function and more detailed characterisation of the function and regulation of specific genes. Approximately 0.7% ... -
Trypanotolerace in West African cattle and the population genetic effects of selection
(Trinity College (Dublin, Ireland). Department of Genetics, 2002)A panel of 69 microsatellite loci was used to investigate the population genetic effects of selection at genes on linked, neutral markers. Loci were typed in African and European Bos taurus cattle and Bos indicus populations ... -
On the role of caspase-recruitment domain proteins in apoptosis and NFkB activation
(Trinity College (Dublin, Ireland). Department of Genetics, 2002)Apoptosis and NFkB activation are two physiological processes that are essential for host mediated response to cellular injury. Apoptosis is a mechanism that removes damaged or unwanted cells. NFkB is a transcription factor ... -
A bioinformatics approach to (intra-) genome comparisons
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2002)The analysis of large volumes of genomic data generates special computational needs. A Beowulf-type computer cluster was set up for high-performance computing. Improvements over existing tools for the efficient ... -
Evolution of vertebrate genome organisation
(Trinity College (Dublin, Ireland). Department of Genetics, 2002)The increasing availability of genomic sequences from different vertebrate organisms affords molecular biologists the opportunity to thoroughly investigate phenomena that were only hinted at by more sparse data. The work ... -
Fourfold faster rate of genome rearrangement in nematodes than in Drosophila
(2002)We compared the genome of the nematode Caenorhabditis elegans to 13% of that of Caenorhabditis briggsae, identifying 252 conserved segments along their chromosomes. We detected 517 chromosomal rearrangements, with the ratio ... -
Sensitivity of Photoreceptor-Derived Cell Line (661W) to Baculoviral p35, Z-VAD.FMK, and Fas-Associated Death Domain
(Association for Research in Vision and Ophthalmology, 2002)PURPOSE: Rod, cone, cone-rod, and macular dystrophies eventually bring about the death of cone photoreceptor cells. The present study explores means of inhibiting apoptosis in addition to inducing a specific apoptotic ... -
Validation in mesenchymal progenitor cells of a mutation-independent ex vivo approach to gene therapy for osteogenesis imperfecta
(Oxford University Press, 2002)Over 100 dominant-negative mutations within the COL1A1 gene have been identified in osteogenesis imperfecta (OI). In terms of human therapeutics, targeting each of these mutations independently is unlikely to be feasible. ...