| dc.contributor.author | HEGNER, MARTIN | en |
| dc.date.accessioned | 2014-02-24T12:39:02Z | |
| dc.date.available | 2014-02-24T12:39:02Z | |
| dc.date.issued | 2012 | en |
| dc.date.submitted | 2012 | en |
| dc.identifier.citation | Jensen, J., Farina, M., Zuccheri, G., Grange, W. and Hegner, M., Quantitative, Label-Free Detection of the Aggregation of alpha-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment, Journal of Sensors, 2012, 2012, Article number 874086 | en |
| dc.identifier.other | Y | en |
| dc.identifier.uri | http://hdl.handle.net/2262/68137 | |
| dc.description | PUBLISHED | en |
| dc.description | Protein aggregation as a cause for neurodegenerative diseases | en |
| dc.description.abstract | Early detection of protein aggregation is of great importance in the field of neurodegenerative diseases. The successful detection of the aggregation of the protein ?-synuclein in a quantitative, label-free manner by functionalising a microcantilever with ?-synuclein monomers and operating it in dynamic mode in the presence of ?-synuclein monomers in solution is reported. A total mass of 6?ng of ?-synuclein was detected over 9 hours on the surface of the cantilever. The result is compared to conventional fluorescence measurements of ?-synuclein aggregation under similar conditions. It is found that the label-free cantilever detection method requires a concentration of protein 50 times smaller than that of the current method and indicated potential for significantly faster response times. | en |
| dc.description.sponsorship | The authors would like to thank the School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, for the use of the FLUOstar Optima microplate multidetection reader. This work was supported by Science Foundation Ireland under the CSET scheme SFI08/CE/I1432 and PI scheme SFI/09IN/1B2623. | en |
| dc.format.extent | Article number 874086 | en |
| dc.language.iso | en | en |
| dc.relation.ispartofseries | Journal of Sensors | en |
| dc.relation.ispartofseries | 2012 | en |
| dc.rights | Y | en |
| dc.subject | protein aggregation | en |
| dc.title | Quantitative, Label-Free Detection of the Aggregation of alpha-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment | en |
| dc.type | Journal Article | en |
| dc.type.supercollection | scholarly_publications | en |
| dc.type.supercollection | refereed_publications | en |
| dc.identifier.peoplefinderurl | http://people.tcd.ie/hegnerm | en |
| dc.identifier.rssinternalid | 89804 | en |
| dc.identifier.doi | http://dx.doi.org/10.1155/2012/874086 | en |
| dc.rights.ecaccessrights | OpenAccess | |
| dc.subject.TCDTheme | Ageing | en |
| dc.subject.TCDTheme | Nanoscience & Materials | en |
| dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
| dc.contributor.sponsorGrantNumber | SFI08/CE/I1432 | en |
| dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
| dc.contributor.sponsorGrantNumber | SFI/09IN/1B2623 | en |
