Quantitative, Label-Free Detection of the Aggregation of alpha-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment
Citation:
Jensen, J., Farina, M., Zuccheri, G., Grange, W. and Hegner, M., Quantitative, Label-Free Detection of the Aggregation of alpha-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment, Journal of Sensors, 2012, 2012, Article number 874086Download Item:
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Abstract:
Early detection of protein aggregation is of great importance in the field of neurodegenerative diseases. The successful detection of the aggregation of the protein ?-synuclein in a quantitative, label-free manner by functionalising a microcantilever with ?-synuclein monomers and operating it in dynamic mode in the presence of ?-synuclein monomers in solution is reported. A total mass of 6?ng of ?-synuclein was detected over 9 hours on the surface of the cantilever. The result is compared to conventional fluorescence measurements of ?-synuclein aggregation under similar conditions. It is found that the label-free cantilever detection method requires a concentration of protein 50 times smaller than that of the current method and indicated potential for significantly faster response times.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
SFI08/CE/I1432
Science Foundation Ireland (SFI)
SFI/09IN/1B2623
Author's Homepage:
http://people.tcd.ie/hegnermDescription:
PUBLISHEDProtein aggregation as a cause for neurodegenerative diseases
Author: HEGNER, MARTIN
Type of material:
Journal ArticleCollections:
Series/Report no:
Journal of Sensors2012
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Full text availableKeywords:
protein aggregationSubject (TCD):
Ageing , Nanoscience & MaterialsDOI:
http://dx.doi.org/10.1155/2012/874086Licences: