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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/64094

Title: Cytoskeletal re-arrangement in TGF-beta1-induced alveolar epithelial-mesenchymal transition studied by atomic force microscopy and high-content analysis
Author: DAVIES, ANTHONY
MEDINA MARTIN, CARLOS
EHRHARDT, CARSTEN
Sponsor: Irish Research Council for Science Engineering and Technology
Author's Homepage: http://people.tcd.ie/ehrhardc
http://people.tcd.ie/amitche
http://people.tcd.ie/martinc2
Keywords: Clinical medicine
Respiratory systems
Epithelial-mesenchymal transition (EMT)
Pulmonary fibrosis
Actin fibres
extracellular matrix
scanning probe microscopy
A549 cell line
Issue Date: 2012
Publisher: Elsevier
Citation: ST Buckley, C Medina, AM Davies, C Ehrhardt, Cytoskeletal re-arrangement in TGF-beta1-induced alveolar epithelial-mesenchymal transition studied by atomic force microscopy and high-content analysis, Nanomed - Nanotechnol Biol Med, 8, 3, 2012, 355-364
Series/Report no.: Nanomedicine: Nanotechnology, Biology and Medicine;8, 3
Abstract: Epithelial-mesenchymal transition (EMT) is closely implicated in the pathogenesis of idiopathic pulmonary fibrosis. Associated with this phenotypic transition is acquisition of an elongated cell morphology and establishment of stress fibres. The extent to which these EMT-associated changes influence cellular mechanics is unclear. We assessed the bio-mechanical properties of alveolar epithelial cells (A549) following exposure to TGF-β1. Using atomic force microscopy, changes in cell stiffness and surface membrane features were determined. Stimulation with TGF-β1 gave rise to a significant increase in stiffness, which was augmented by a collagen I matrix. Additionally, TGF-β1-treated cells exhibited a rougher surface profile with notable protrusions. Simultaneous quantitative examination of the morphological attributes of stimulated cells using an image-based high-content analysis system revealed dramatic alterations in cell shape, F-actin content and distribution. Together, these investigations point to a strong correlation between the cytoskeletal-associated cellular architecture and the mechanical dynamics of alveolar epithelial cells undergoing EMT.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/64094
Related links: http://dx.doi.org/10.1016/j.nano.2011.06.021
Appears in Collections:Pharmacy and Pharmaceutical Sciences (Scholarly Publications)

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