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Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/59846

Title: Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays
Author: O'DONNELL, JAMES
Sponsor: 
Name Grant Number
TD/2009/0124

Author's Homepage: http://people.tcd.ie/jodonne
Keywords: Hematology
human plasma
Issue Date: 2011
Publisher: Elsevier
Citation: Vanessa Castro-Lopez, Leanne F. Harris, James S. O'Donnell, Anthony J. Killard, Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays, Journal of Pharmaceutical and Biomedical Analysis, 56, 5, 2011, 992-997
Series/Report no.: Journal of Pharmaceutical and Biomedical Analysis;
56;
5;
Abstract: Novel and sensitive plate-based fluorogenic anti-factor Xa (FXa) assays were investigated to quantify unfractionated heparin (UFH) in human plasma and whole blood within the therapeutic ranges of 0-1.6 U/mL and 0–0.8 U/mL, respectively. Two fluorogenic anti-FXa assay methods were defined for low (0-0.6 U/mL) and high (0.6-1.2 U/mL) pharmacologically relevant UFH concentration ranges in pooled human plasma. In both cases significant differences were observed at intervals of 0.2 U/mL (P < 0.05). The semi-logarithmic plots of the calibration curves in the low and high UFH range were both fitted to linear regressions with correlation coefficients of 0.96 and >0.99, respectively. The assay was also optimized for whole blood which was capable of differentiating UFH concentrations at intervals of 0.2 U/mL (P < 0.05) in the range of 0-0.4 U/mL. The statistically different results were fitted to a linear regression with a correlation coefficient of >0.99. The results obtained in this study could assist diagnostic laboratories towards improved monitoring of UFH therapy.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/59846
Related links: http://dx.doi.org/10.1016/j.jpba.2011.07.025
Appears in Collections:Haematology (Scholarly Publications)

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