Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap.
Item Type:Journal Article
Citation:Bazou D, Kearney R, Mansergh F, Bourdon C, Farrar J, Wride M, Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap., Ultrasound in medicine & biology, 37, 2, 2011, 321-30
Gene Expression Analysis of Mouse Embryonic Stem Cells Following Levitation in an Ultrasound Standing Wave Trap.pdf (Published (publisher's copy) - Peer Reviewed) 702.0Kb
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08?0.85 MPa) and times (5?60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine.
MTKD-CT-2006- 042519 - Nanofab
Health Research Board (HRB)
Type of material:Journal Article
Series/Report no:Ultrasound in medicine & biology
Availability:Full text available