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dc.contributor.authorPIDGEON, GRAHAM
dc.date.accessioned2010-02-01T09:48:33Z
dc.date.available2010-02-01T09:48:33Z
dc.date.issued2006
dc.date.submitted2006en
dc.identifier.citationCryan LM, Pidgeon GP, Fitzgerald DJ, O'Brien CJ. `COX-2 protects against thrombosis of the retinal vasculature in a mouse model of proliferative retinopathy? in Molecular Vision, 12, 2006, pp 405 - 414en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/36609
dc.descriptionPUBLISHEDen
dc.description.abstractPurpose: Cyclooxygenases (COX-1 and COX-2) and prostaglandins regulate angiogenesis in several settings, including cancer and ischemia. In the eye, both selective inhibitors of COX-2 and nonselective COX inhibitors are reported to suppress ischemia-related retinal angiogenesis. Such studies however, may be confounded by the nonspecific effects of inhibitors. Methods: Mice lacking either the COX-1 (COX-1-/-) or COX-2 isoform (COX-2-/-) were employed in a model of oxygeninduced retinopathy. Vascular responses were examined by histology, isolectin B4 staining of the abluminal endothelium, and retinal fluorescein angiography. Results: There was an increase in intravitreal endothelial nuclei in hyperoxia-treated mice compared to normoxic controls irrespective of the genotype. Quantitative analysis of fluorescein-perfused and isolectin B4-stained retinal angiograms at postnatal day 18 (P18) revealed similar global levels of neovascular tufts in hyperoxia-treated wild-type, COX-1-/-, and COX-2-/- mice. However, hyperoxia-treated COX-2-/- mice had increased areas of retinal nonperfusion (29.2?1.9 compared to 16.3?2.7; n=6; p<0.001). COX-1 disruption had no effect (15.6?2.6; n=8). Platelet deposition within retinal vessels was increased in hyperoxia-treated COX-2-/- mice (p<0.05). Conclusions: Genetic disruption of a single COX isoform is not sufficient to prevent oxygen-induced retinopathy. COX- 2 protects retinal vessels from thrombosis, limiting the area of retinal nonperfusion in oxygen-induced retinopathy.en
dc.format.extent405en
dc.format.extent414en
dc.format.extent314512 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherMolecular Visionen
dc.relation.ispartofseriesMolecular Visionen
dc.relation.ispartofseries12en
dc.rightsYen
dc.subjectSurgery
dc.titleCOX-2 protects against thrombosis of the retinal vasculature in a mouse model of proliferative retinopathy.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pidgeong
dc.identifier.rssinternalid42879
dc.identifier.rssurihttp://www.molvis.org/molvis/v12/a47/en


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