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dc.contributor.authorCitiulo, Francesco
dc.contributor.authorMoran, Gary
dc.contributor.authorCOLEMAN, DAVID
dc.contributor.authorSULLIVAN, DEREKen
dc.date.accessioned2009-06-30T13:47:52Z
dc.date.available2009-06-30T13:47:52Z
dc.date.issued2009-06-30T13:47:52Z
dc.date.issued2009en
dc.date.submitted2009en
dc.identifier.citationCitiulo, F., Moran, G.P., Coleman, D.C. and Sullivan, D.J. 'Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media' in FEMS Yeast Research, 9, (7), 2009, pp 1051 - 1060en
dc.identifier.otherYen
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/31046
dc.descriptionPUBLISHEDen
dc.description.abstractCandida albicans and Candida dubliniensis are the only Candida species that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are greater than 30 days old. However, 5-15 day old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were pre-induced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialised form of growth by C. albicans and C. dubliniensis.en
dc.description.sponsorshipEU (MRTN-CT-2004-512481 (CanTrain)) Dublin Dental School & Hospitalen
dc.format.extent153088 bytes
dc.format.extent1051en
dc.format.extent1060en
dc.format.mimetypeapplication/msword
dc.language.isoenen
dc.relation.ispartofseries9en
dc.relation.ispartofseries7en
dc.relation.ispartofseriesFEMS Yeast Researchen
dc.rightsYen
dc.subjectCandida albicansen
dc.subjectChlamydosporeen
dc.subjectCandida dubliniensisen
dc.titlePurification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid mediaen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/djsullvnen
dc.identifier.rssinternalid59122en
dc.identifier.rssurihttp://www3.interscience.wiley.com/cgi-bin/fulltext/122414171/PDFSTARTen


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