dc.contributor.author | Citiulo, Francesco | |
dc.contributor.author | Moran, Gary | |
dc.contributor.author | COLEMAN, DAVID | |
dc.contributor.author | SULLIVAN, DEREK | en |
dc.date.accessioned | 2009-06-30T13:47:52Z | |
dc.date.available | 2009-06-30T13:47:52Z | |
dc.date.issued | 2009-06-30T13:47:52Z | |
dc.date.issued | 2009 | en |
dc.date.submitted | 2009 | en |
dc.identifier.citation | Citiulo, F., Moran, G.P., Coleman, D.C. and Sullivan, D.J. 'Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media' in FEMS Yeast Research, 9, (7), 2009, pp 1051 - 1060 | en |
dc.identifier.other | Y | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/31046 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Candida albicans and Candida dubliniensis are the only Candida species that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are greater than 30 days old. However, 5-15 day old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were pre-induced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialised form of growth by C. albicans and C. dubliniensis. | en |
dc.description.sponsorship | EU (MRTN-CT-2004-512481 (CanTrain))
Dublin Dental School & Hospital | en |
dc.format.extent | 153088 bytes | |
dc.format.extent | 1051 | en |
dc.format.extent | 1060 | en |
dc.format.mimetype | application/msword | |
dc.language.iso | en | en |
dc.relation.ispartofseries | 9 | en |
dc.relation.ispartofseries | 7 | en |
dc.relation.ispartofseries | FEMS Yeast Research | en |
dc.rights | Y | en |
dc.subject | Candida albicans | en |
dc.subject | Chlamydospore | en |
dc.subject | Candida dubliniensis | en |
dc.title | Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/djsullvn | en |
dc.identifier.rssinternalid | 59122 | en |
dc.identifier.rssuri | http://www3.interscience.wiley.com/cgi-bin/fulltext/122414171/PDFSTART | en |