dc.contributor.author | FOSTER, TIMOTHY | |
dc.date.accessioned | 2020-08-07T14:26:05Z | |
dc.date.available | 2020-08-07T14:26:05Z | |
dc.date.issued | 1993 | |
dc.date.submitted | 1993 | en |
dc.identifier.citation | McDevitt, D., Wann, E.R., Foster, T.J., Recombination at the coagulase locus in Staphylococcus aureus: plasmid integration and amplification, Journal of General Microbiology, 1993, 139, 4, 695 - 706 | en |
dc.identifier.other | Y | |
dc.identifier.other | doi: 10.1099/00221287-139-4-695 | |
dc.identifier.uri | http://hdl.handle.net/2262/93136 | |
dc.description.abstract | The integrating plasmid pCOA18, comprising pUC18 linked to a mutated coagulase (coa) gene from Staphylococcus aureus, and constructed by substituting coa sequences with a tetracycline (Tc)-resistance marker (delta coa::Tcr), was transformed into Staphylococcus aureus RN4220, where it underwent recombination with the chromosomal coa locus. Allele-replacement mutants were recovered at a low frequency directly after transformation. The majority of transformants carried pCOA18 integrated in the chromosome by a single Campbell-type recombination event. The majority of integrants contained tandem repeats of pCOA18 and expressed high levels of resistance to Tc (> 30 micrograms ml-1) compared to the single-copy integrants and allele-replacement mutants (15 micrograms ml-1). Transduction of a single-copy integrant to a Coa+ recipient allowed the cointegrant to be resolved and allele-replacement recombinants to be selected. In addition, growth of a single-copy integrant on high concentrations of Tc (> 30 micrograms ml-1) selected for amplified derivatives at a frequency of 10(-5). It was estimated that up to 19 copies of pCOA18 could occur in a tandem array in the chromosome. | en |
dc.format.extent | 695 | en |
dc.format.extent | 706 | en |
dc.format.extent | <a title= 'Click on this link to see the details for this article.' href='http://tcdlocalportal.tcd.ie/pls/webapps/cerif.cerif_publications_isi.show_article?p_article_id=83192403 '>ISI article no: 83192403</a> | en |
dc.format.medium | ISI_83192403 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Journal of General Microbiology; | |
dc.relation.ispartofseries | 139; | |
dc.relation.ispartofseries | 4; | |
dc.rights | Y | en |
dc.title | Recombination at the coagulase locus in Staphylococcus aureus: plasmid integration and amplification | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/tfoster | |
dc.identifier.rssinternalid | 1851 | |
dc.rights.ecaccessrights | openAccess | |
dc.identifier.rssuri | http://mic.sgmjournals.org/cgi/reprint/139/4/695?view=long&pmid=8515230 | |