Recombination and complementation between R factors in Escherichia coli K12
Citation:
Foster, T.J. and Howe, T.G.B., Recombination and complementation between R factors in Escherichia coli K12, Genetical Research Cambridge, 1971, 18, 287 - 297Download Item:
Abstract:
Recombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated in Escherichia coli K12 rec+; it occurs at reduced frequency in recB and recC, and is not detectable in reeA, indicating that R factor recombination depends on host functions. Some mutants of R1 also recombine with an R100 mutant in a similar way. recA cells carrying an R1 and an R100 Cms mutant (hetero-R state) have a low level of chloramphenicol-resistance, and form a chloramphenicol acetyl transferase that has lower specific activity than enzyme from hosts carrying wild-type or recombinant factors. These results suggest the occurrence of interallelic complementation between mutant R factors.
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http://people.tcd.ie/tfosterDescription:
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Author: FOSTER, TIMOTHY
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Genetical Research, Cambridge;18;
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http://dx.doi.org/10.1017/S0016672300012696Licences: