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dc.contributor.authorCAFFREY, MARTINen
dc.date.accessioned2011-02-01T18:15:11Z
dc.date.available2011-02-01T18:15:11Z
dc.date.issued2010en
dc.date.submitted2010en
dc.identifier.citationHöfer N, Aragão D, Caffrey M, Crystallizing Transmembrane Peptides in Lipidic Mesophases, Biophysical Journal, 99, 3, 2010, L23-L25en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/49865
dc.descriptionPUBLISHEDen
dc.description.abstractStructure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally implemented, would not apply to small protein targets having ?4 transmembrane crossings. In our study, the hypothesis that the inherent flexibility of the mesophase would enable crystallogenesis of small proteins was tested using a transmembrane pentadecapeptide, linear gramicidin, which produced structure-grade crystals. This result suggests that the in meso method should be considered as a viable means for high-resolution structure determination of integral membrane peptides, many of which are predicted to be coded for in the human genome.en
dc.description.sponsorshipThis work was supported by the Science Foundation Ireland (grant No. 07/ IN.1/B1836), FP7 COST Action (grant No. CM0902), and the National Institutes of Health (No. GM75915). Use of the Advanced Photon Source is supported by the US Department of Energy (grant No. DE-AC02- 06CH11357). Diffraction data were collected at the GM/CA-CAT beamline, Advanced Photon Source; GM/CA-CAT is funded by the US National Institutes of Cancer (grant No. Y1-CO-1020) and General Medical Sciences (grant No. Y1-GM-1104).en
dc.format.extentL23-L25en
dc.language.isoenen
dc.relation.ispartofseriesBiophysical Journalen
dc.relation.ispartofseries99en
dc.relation.ispartofseries3en
dc.rightsYen
dc.subjectBiochemistryen
dc.subjectPeptidesen
dc.subjectmembrane proteinsen
dc.titleCrystallizing Transmembrane Peptides in Lipidic Mesophasesen
dc.typeJournal Articleen
dc.contributor.sponsorNational Institutes of Health (NIH)en
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcaffreen
dc.identifier.rssinternalid70496en
dc.relation.ecprojectidinfo:eu-repo/grantAgreement/EC/FP7/07/IN.1/B1836
dc.rights.ecaccessrightsOpenAccess
dc.contributor.sponsorGrantNumberGM75915en
dc.contributor.sponsorGrantNumberCM0902en
dc.contributor.sponsorGrantNumber07/IN.1/B1836en
dc.identifier.rssurihttp://dx.doi.org/10.1016/j.bpj.2010.05.011en


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