dc.contributor.author | CAFFREY, MARTIN | en |
dc.date.accessioned | 2011-02-01T18:15:11Z | |
dc.date.available | 2011-02-01T18:15:11Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Höfer N, Aragão D, Caffrey M, Crystallizing Transmembrane Peptides in Lipidic Mesophases, Biophysical Journal, 99, 3, 2010, L23-L25 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/49865 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally implemented, would not apply to small protein targets having ?4 transmembrane crossings. In our study, the hypothesis that the inherent flexibility of the mesophase would enable crystallogenesis of small proteins was tested using a transmembrane pentadecapeptide, linear gramicidin, which produced structure-grade crystals. This result suggests that the in meso method should be considered as a viable means for high-resolution structure determination of integral membrane peptides, many of which are predicted to be coded for in the human genome. | en |
dc.description.sponsorship | This work was supported by the Science Foundation Ireland (grant No. 07/
IN.1/B1836), FP7 COST Action (grant No. CM0902), and the National
Institutes of Health (No. GM75915). Use of the Advanced Photon Source
is supported by the US Department of Energy (grant No. DE-AC02-
06CH11357). Diffraction data were collected at the GM/CA-CAT beamline,
Advanced Photon Source; GM/CA-CAT is funded by the US National
Institutes of Cancer (grant No. Y1-CO-1020) and General Medical Sciences
(grant No. Y1-GM-1104). | en |
dc.format.extent | L23-L25 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Biophysical Journal | en |
dc.relation.ispartofseries | 99 | en |
dc.relation.ispartofseries | 3 | en |
dc.rights | Y | en |
dc.subject | Biochemistry | en |
dc.subject | Peptides | en |
dc.subject | membrane proteins | en |
dc.title | Crystallizing Transmembrane Peptides in Lipidic Mesophases | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/mcaffre | en |
dc.identifier.rssinternalid | 70496 | en |
dc.relation.ecprojectid | info:eu-repo/grantAgreement/EC/FP7/07/IN.1/B1836 | |
dc.rights.ecaccessrights | OpenAccess | |
dc.identifier.rssuri | http://dx.doi.org/10.1016/j.bpj.2010.05.011 | en |
dc.contributor.sponsor | National Institutes of Health (NIH) | en |
dc.contributor.sponsorGrantNumber | GM75915 | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.sponsorGrantNumber | CM0902 | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsorGrantNumber | 07/IN.1/B1836 | en |