Browsing Biochemistry (Scholarly Publications) by Sponsor "National Institutes of Health (NIH)"
Now showing items 1-17 of 17
-
Acute Inflammation Alters Brain Energy Metabolism in Mice and Humans: Role in Suppressed Spontaneous Activity, Impaired Cognition, and Delirium
(2020)Systemic infection triggers a spectrum of metabolic and behavioral changes, collectively termed sickness behavior, which while adaptive, can affect mood and cognition. In vulnerable individuals, acute illness can also ... -
Comprehensive characterization of cytochrome P450 isozyme selectivity across chemical libraries.
(Nature Publishing Group, 2009)The cytochrome P450 (CYP) gene family catalyzes drug metabolism and bioactivation and is therefore relevant to drug development. We determined potency values for 17,143 compounds against five recombinant CYP isozymes (1A2, ... -
Crystal structure of the integral membrane diacylglycerol kinase
(2013)Diacylglycerol kinase catalyses the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid for use in shuttling water-soluble components to membrane-derived oligosaccharide and lipopolysaccharide in the cell ... -
Crystal structure of the Toll/interleukin-1 receptor domain of human IL-1RAPL
(The American Society for Biochemistry and Molecular Biology, 2004)The Toll/interleukin-1 receptor (TIR) domain is conserved in the intracellular regions of Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) as well as in several cytoplasmic adapter molecules. This domain has ... -
Crystallizing membrane proteins using lipidic mesophases.
(2009)A detailed protocol for crystallizing membrane proteins that makes use of lipidic mesophases is described. This has variously been referred to as the lipid cubic phase or in meso method. The method has been shown to be ... -
Crystallizing Transmembrane Peptides in Lipidic Mesophases
(2010)Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally ... -
Direct correlation of structure changes and thermal events in hydrated lipid established by simultaneous calorimetry and time-resolved x-ray diffraction.
(1992)In many lipid systems, polymorphic and mesomorphic behavior depends on sample thermal history. To establish unequivocally the structural origin of endothermic and exothermic events in such systems, we have performed ... -
A fast, simple and robust protocol for growing crystals in the lipidic cubic phase
(2012)A simple and inexpensive protocol for producing crystals in the sticky and viscous mesophase used for membrane protein crystallization by the in meso method is described. It provides crystals that appear within 15–30 min ... -
Folate/homocysteine phenotypes and MTHFR 677C>T genotypes are associated with serum levels of monocyte chemoattractant protein-1.
(Elsevier, 2009)Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that recruits monocytes into the subendothelial cell layer in atherosclerotic lesions. Elevated homocysteine (hyperhomocysteinemia), which is usually associated ... -
Genetic and nutritional factors contributing to hyperhomocysteinemia in young adults
(American Society of Hematology, 2003)A modestly elevated total plasma homocysteine concentration (tHcy) is generally accepted as an independent and graded risk factor for various pathologies, including vascular diseases, neural tube defects, Alzheimer disease, ... -
Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
(2011)The lipidic cubic mesophase has been used to crystallize important membrane proteins for high-resolution structure determination. To date, however, no integral membrane enzymes have yielded to this method, the in meso. For ... -
Macrophage fumarate hydratase restrains mtRNA-mediated interferon production.
(Springer Nature, 2023-03-08)Metabolic rewiring underlies the effector functions of macrophages, but the mechanisms involved remain incompletely defined. Here, using unbiased metabolomics and stable isotope-assisted tracing, we show that an inflammatory ... -
Resolution of inflammation: state of the art, definitions and terms
(The Federation of American Societies for Experimental Biology., 2006)A recent focus meeting on Controlling Acute Inflammation was held in London, April 27-28, 2006, organized by D.W. Gilroy and S.D. Brain for the British Pharmacology Society. We concluded at the meeting that a consensus ... -
Th1-Th2 polarisation and autophagy in the control of intracellular mycobacteria by macrophages
(Elsevier, 2009)Autophagy is a recently recognized immune effector mechanism against intracellular pathogens. The role of autophagy in innate immunity has been well established, but the extent of its regulation by the adaptive immune ... -
Two new and rapid approaches for studying the phase properties of cosmetic lipids and oils
(Society of Cosmetic Chemists, 1988)Lipids and oils, integral components of cosmetic products, exist in a number of intermediate physical states, or mesomorphic phases, between the crystalline solid and isotropic liquid. The stability of these phases depends ... -
Tyrosine phosphorylation of MyD88 adapter-like (Mal) is critical for signal transduction and blocked in endotoxin tolerance
(American Society for Biochemistry and Molecular Biology, 2008)Toll-like receptor 4 (TLR4) recognition of lipopolysaccharide triggers signalosome assembly among TLR4, sorting (e.g. MyD88 adapter-like (Mal)) and signaling (e.g. MyD88) adapters, initiating recruitment and activation of ...