Browsing Biochemistry (Scholarly Publications) by Author "Kelly, Vincent"
Now showing items 1-9 of 9
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CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not regulate nuclear transcription, but is expressed in macrophages
Carty, Michael; Kelly, Vincent; Bowie, Andrew; Hokamp, Karsten (2021)SARM1 is a toll/interleukin-1 receptor -domain containing protein, with roles proposed in both innate immunity and neuronal degeneration. Murine SARM1 has been reported to regulate the transcription of chemokines in both ... -
The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi?bi mechanism
Southern, John; Kelly, Vincent; Connon, Stephen (2020)Eukaryotic tRNA-guanine transglycosylase (TGT) – an enzyme recently recognised to be of potential therapeutic importance – catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with ... -
Heterozygous disruption of ALAS1 in mice causes an accelerated age-dependent reduction in free heme, but not total heme, in skeletal muscle and liver
Kelly, Vincent (2021)5-Aminolevulinic acid (ALA) is the rate-limiting intermediate in heme biosynthesis in vertebrate species; a re- action catalyzed by the mitochondrial ALA synthase 1 (ALAS1) enzyme. Previously we reported that knockdown of ... -
The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity.
Kelly, Vincent; Senge, Mathias; Southern, John; Hokamp, Karsten; Connon, Stephen (2021)Base-modification can occur throughout a transfer RNA molecule; however, elaboration is particularly prevalent at position 34 of the anticodon loop (the wobble position), where it functions to influence protein translation. ... -
Malonylation of GAPDH is an inflammatory signal in macrophages
O'Neill, Luke; Kelly, Vincent; Palsson Mcdermott, Eva (2019)Macrophages undergo metabolic changes during activation that are coupled to functional responses. The gram negative bacterial product lipopolysaccharide (LPS) is especially potent at driving metabolic reprogramming, enhancing ... -
Next generation SARM1 knockout and epitope tagged CRISPR-Cas9-generated isogenic mice reveal that SARM1 does not participate in regulating nuclear transcription, despite confirmation of protein expression in macrophages
Kelly, Vincent (2021)ABSTRACT SARM1 is an ancient and highly conserved TIR-domain containing protein, with a diverse range of proposed roles in both innate immunity and neuronal death and degeneration. Murine SARM1 has been reported to ... -
A non-toxic, reversibly released imaging probe for oral cancer that is derived from natural compounds.
Kelly, Vincent; Ghanim, Magda; Mc Manus, Gavin; Mok, Kenneth (2021)CD44 is emerging as an important receptor biomarker for various cancers. Amongst these is oral cancer, where surgical resection remains an essential mode of treatment. Unfortunately, surgery is frequently associated with ... -
Queuine micronutrient deficiency promotes warburg metabolism and reversal of the mitochondrial ATP synthase in hela cells
Kelly, Vincent; Nolan, Derek (2020)ueuine is a eukaryotic micronutrient, derived exclusively from eubacteria. It is incorporated into both cytosolic and mitochondrial transfer RNA to generate a queuosine nucleotide at position 34 of the anticodon loop. The ... -
Spiroindoline-Capped Selective HDAC6 Inhibitors: Design, 2 Synthesis, Structural Analysis, and Biological Evaluation
Zisterer, Daniela; Kelly, Vincent; O'Sullivan, Jeffrey (2020)Histone deacetylase inhibitors (HDACi) have emerged as promising therapeutics for the treatment of neurodegeneration, cancer, and rare disorders. Herein, we report the development of a series of spiroindoline-based HDAC6 ...