Browsing Biochemistry (Theses and Dissertations) by Author "Breen, Eamon Patrick"
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Detection, expression and elucidation of the mechanism of action of mitochondrial uncoupling Protein 1
Breen, Eamon Patrick (Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)A system for overexpression of recombinant proteins in E. coli is defined. Using subcloning techniques the cDNAs for human uncoupling proteins (hUCP) 1, 2 and 3 were introduced to the pET expression system for E. coli. ...