The development of a three-dimensional scaffold for ex vivo biomimicry of human acute myeloid leukaemia
Citation:
Blanco T.M., Mantalaris A., Bismarck A., Panoskaltsis N., The development of a three-dimensional scaffold for ex vivo biomimicry of human acute myeloid leukaemia, Biomaterials, 31, 8, 2010, 2243 - 2251Download Item:
Abstract:
Acute myeloid leukaemia (AML) is a cancer of haematopoietic cells that develops in three-dimensional
(3-D) bone marrow niches in vivo. The study of AML has been hampered by lack of appropriate ex vivo
models that mimic this microenvironment. We hypothesised that fabrication and optimisation of suit-
able biomimetic scaffolds for culturing leukaemic cells ex vivo might facilitate the study of AML in its
native 3-D niche. We evaluated the growth of three leukaemia subtype-specific cell lines, K-562, HL60
and Kasumi-6, on highly porous scaffolds fabricated from biodegradable and non-biodegradable
polymeric materials, such as poly (L-lactic-co-glycolic acid) (PLGA), polyurethane (PU), poly (methyl-
methacrylate), poly ( D, L-lactade), poly (caprolactone), and polystyrene. Our results show that PLGA and
PU supported the best seeding efficiency and leukaemic growth. Furthermore, the PLGA and PU scaffolds
were coated with extracellular matrix (ECM) proteins, collagen type I (62.5 or 125 mg/ml) and fibronectin
(25 or 50 mg/ml) to provide biorecognition signals. The 3 leukaemia subtype-specific lines grew best on
PU scaffolds coated with 62.5 mg/ml collagen type I over 6 weeks in the absence of exogenous growth
factors. In conclusion, PU-collagen scaffolds may provide a practical model to study the biology and
treatment of primary AML in an ex vivo mimicry.
Author's Homepage:
http://people.tcd.ie/panoskanDescription:
PUBLISHED
Author: Panoskaltsis, Nicki
Type of material:
Journal ArticleSeries/Report no:
Biomaterials;31;
8;
Availability:
Full text availableKeywords:
Haematopoiesis, Leukaemia culture, Three-dimensional culture, ScaffoldDOI:
http://dx.doi.org/10.1016/j.biomaterials.2009.11.094ISSN:
01429612Metadata
Show full item recordLicences: