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dc.contributor.authorCOLEMAN, JONATHANen
dc.contributor.authorNICOLOSI, VALERIAen
dc.contributor.authorVOLKOV, YURIen
dc.contributor.authorO'NEILL, LUKEen
dc.contributor.authorMCGOVERN, IGNATIUSen
dc.contributor.authorNERL, HANNAHen
dc.contributor.authorDUESBERG, GEORGen
dc.contributor.authorMCEVOY, NIALLen
dc.date.accessioned2016-09-16T11:06:13Z
dc.date.available2016-09-16T11:06:13Z
dc.date.created2016en
dc.date.issued2016en
dc.date.submitted2016en
dc.identifier.citationMcIntyre J, Verma N.K, Smith R.J, Moore C, Nerl H, McEvoy N, Berner N, McGovern I, Khan U, Lyons P, O'Neill L, Nicolosi V, Duesberg G.S, Byrne H.J, Coleman J, Volkov Y, A comparison of catabolic pathways induced in primary macrophages by pristine single walled carbon nanotubes and pristine graphene, RSC Advances, 6, 70, 2016, 65299 - 65310en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/77354
dc.descriptionPUBLISHEDen
dc.descriptionExport Date: 15 September 2016en
dc.description.abstractUnderstanding the correlation between the physico-chemical properties of carbonaceous nanomaterials and how these properties impact on cells and subcellular mechanisms is critical to their risk assessment and safe translation into newly engineered devices. Here the toxicity, uptake and catabolic response of primary human macrophages to pristine graphene (PG) and pristine single walled carbon nanotubes (pSWCNT) are explored, compared and contrasted. The nanomaterial toxicity was assessed using three complementary techniques (live–dead assay, real time impedance technique and confocal microscopic analysis), all of which indicated no signs of acute cytotoxicity in response to PG or pSWCNT. Transmission electron microscopy (TEM) demonstrated that PG was phagocytosed by the cells into single membrane lysosomal vesicles, whereas the primary macrophages exposed to pSWCNT contained many double membrane vesicles indicative of an autophagic response. These distinct catabolic pathways were further verified by biochemical and microscopic techniques. Raman spectroscopic mapping was used to explore the nanomaterial uptake and distribution. Based on the G-band, significant uptake and accumulation of the PG in discrete vesicles was recorded, whereas the pSWCNT were not taken up to the same extent. Thermogravimetric analysis (TGA) of the cells treated with PG revealed that ∼20–30% of the remaining dry mass was made up of PG. No detectable amount of pSWCNT was recorded using TGA. TEM analysis confirmed that PG was still graphitic even after 24 hours of accumulation in the lysosomal compartments. In conclusion, these two nanomaterials, with similar surface chemistries but unique geometries, differ significantly in their uptake mechanisms and subsequently induced lysosomal and autophagic catabolic pathways in human primary macrophagesen
dc.description.sponsorship08/CE/I1432S; SFI; European Commission 246479; EC; European Commissionen
dc.format.extent65299en
dc.format.extent65310en
dc.relation.ispartofseriesRSC Advancesen
dc.relation.ispartofseries6en
dc.relation.ispartofseries70en
dc.rightsYen
dc.subjectcarbonaceous nanomaterialsen
dc.subject.lcshcarbonaceous nanomaterialsen
dc.titleA comparison of catabolic pathways induced in primary macrophages by pristine single walled carbon nanotubes and pristine grapheneen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/colemajen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/imcgovrnen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/duesbergen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/laoneillen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcevoynien
dc.identifier.peoplefinderurlhttp://people.tcd.ie/yvolkoven
dc.identifier.peoplefinderurlhttp://people.tcd.ie/nicoloven
dc.identifier.peoplefinderurlhttp://people.tcd.ie/nerlhen
dc.identifier.rssinternalid125599en
dc.identifier.doihttp://dx.doi.org/10.1039/c6ra02476aen
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorGrantNumber08/CE/I1432Sen
dc.identifier.rssurihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84978681981&partnerID=40&md5=043d111c7b6c648f6d9e2fc0bf2a8b87en
dc.identifier.orcid_id0000-0001-9659-9721en


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