Non-Invasive Monitoring Of Chick Development In Ovo Using A 7T MRI System From Day 12 Incubation Through To Hatching
Item Type:Journal Article
Citation:Bain M.M., Fagan A.J., Mullin J.M., McNaught I., McLean J. and Condon B, Non-Invasive Monitoring Of Chick Development In Ovo Using A 7T MRI System From Day 12 Incubation Through To Hatching, Journal of Magnetic Resonance Imaging, 26, 1, 2007, 198 - 201
Bain 2007 JMRI - Tara.pdf (Accepted for publication (author's copy) - Peer Reviewed) 245.8Kb
Purpose: To determine whether mild cooling of the egg reduces movement to the point where an ultra-high-field (7T) MRI system can be used to noninvasively monitor chick growth in ovo from 12 days incubation through to hatching. Materials and Methods: Group A eggs were incubated at 37.5?C for 21 days. Group B eggs were removed from the incubator on days 12, 15, 17, 18, 19, and 20 of incubation, cooled for one hour, and then returned to the incubator. Group C eggs were cooled as for group B and then individually imaged for 25 minutes using a 7T MRI system before being returned to the incubator. The average size (volume) of the heart, liver, and brain at each stage of incubation was estimated from the T2-weighted images and compared with existing values in the literature. Results: The combination of cooling and MRI significantly reduced chick movement to allow excellent image acquisition at each stage of incubation. Repeated cooling and/or MRI did not significantly slow down or arrest the development of the chicks in either of the experimental groups. Conclusion: MRI provides a powerful noninvasive tool to study chick development and the growth of individual organs, including the brain, liver, and heart, in ovo from 12 days? incubation.
Type of material:Journal Article
Series/Report no:Journal of Magnetic Resonance Imaging;
Availability:Full text available