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dc.contributor.authorHEGNER, MARTINen
dc.date.accessioned2010-04-22T15:36:39Z
dc.date.available2010-04-22T15:36:39Z
dc.date.issued2008en
dc.date.submitted2008en
dc.identifier.citationGhatkesar, M. K., Lang, H. P., Gerber, C., Hegner, M., Braun, T., Comprehenisive characterization of molecular interactions based on nanomechanics, PLoS ONE, 3, 11, 2008, e3610en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/39177
dc.descriptionPUBLISHEDen
dc.description.abstractMolecular interaction is a key concept in our understanding of the biological mechanisms of life. Two physical properties change when one molecular partner binds to another. Firstly, the masses combine and secondly, the structure of at least one binding partner is altered, mechanically transducing the binding into subsequent biological reactions. Here we present a nanomechanical micro-array technique for bio-medical research, which not only monitors the binding of effector molecules to their target but also the subsequent effect on a biological system in vitro. This label-free and real-time method directly and simultaneously tracks mass and nanomechanical changes at the sensor interface using micro-cantilever technology. T1o prove the concept we measured lipid vesicle (~748*106 Da) adsorption on the sensor interface followed by subsequent binding of the bee venom peptide melittin (2840 Da) to the vesicles. The results show the high dynamic range of the instrument and that measuring the mass and structural changes simultaneously allow a comprehensive discussion of molecular interactions.en
dc.description.sponsorshipFinancial support is acknowledged from SNF (NCCR Nanoscale Science, Switzerland), the Cleven-Becker Stiftung, Endress Foundation (Switzerland), and the ELTEM Regio and SFI (Ireland). Author MKG would like to thank Swiss National Science Foundation (SNSF) and Novartis Foundation, Switzerland for the research fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.format.extente3610en
dc.language.isoenen
dc.relation.ispartofseriesPLoS ONEen
dc.relation.ispartofseries3en
dc.relation.ispartofseries11en
dc.rightsYen
dc.subjectPhysics
dc.titleComprehenisive characterization of molecular interactions based on nanomechanicsen
dc.typeJournal Articleen
dc.contributor.sponsorNational Science Foundation
dc.contributor.sponsorScience Foundation Irelanden
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/hegnermen
dc.identifier.rssinternalid64916en


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