dc.contributor.author | HEGNER, MARTIN | en |
dc.date.accessioned | 2010-04-22T15:36:39Z | |
dc.date.available | 2010-04-22T15:36:39Z | |
dc.date.issued | 2008 | en |
dc.date.submitted | 2008 | en |
dc.identifier.citation | Ghatkesar, M. K., Lang, H. P., Gerber, C., Hegner, M., Braun, T., Comprehenisive characterization of molecular interactions based on nanomechanics, PLoS ONE, 3, 11, 2008, e3610 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/39177 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Molecular interaction is a key concept in our understanding of the biological mechanisms of life. Two physical properties change when one molecular partner binds to another. Firstly, the masses combine and secondly, the structure of at least one binding partner is altered, mechanically transducing the binding into subsequent biological reactions. Here we present a nanomechanical micro-array technique for bio-medical research, which not only monitors the binding of effector molecules to their target but also the subsequent effect on a biological system in vitro. This label-free and real-time method directly and simultaneously tracks mass and nanomechanical changes at the sensor interface using micro-cantilever technology. T1o prove the concept we measured lipid vesicle (~748*106 Da) adsorption on the sensor interface followed by subsequent binding of the bee venom peptide melittin (2840 Da) to the vesicles. The results show the high dynamic range of the instrument and that measuring the mass and structural changes simultaneously allow a comprehensive discussion of molecular interactions. | en |
dc.description.sponsorship | Financial support is acknowledged from SNF (NCCR Nanoscale Science, Switzerland), the Cleven-Becker Stiftung, Endress Foundation (Switzerland), and
the ELTEM Regio and SFI (Ireland). Author MKG would like to thank Swiss National Science Foundation (SNSF) and Novartis Foundation, Switzerland for the
research fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en |
dc.format.extent | e3610 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | PLoS ONE | en |
dc.relation.ispartofseries | 3 | en |
dc.relation.ispartofseries | 11 | en |
dc.rights | Y | en |
dc.subject | Physics | |
dc.title | Comprehenisive characterization of molecular interactions based on nanomechanics | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/hegnerm | en |
dc.identifier.rssinternalid | 64916 | en |
dc.contributor.sponsor | National Science Foundation | |
dc.contributor.sponsor | Science Foundation Ireland | en |