Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination
Citation:Rakovich, Y.P.; Yang, L.; Taylor, C.M.; Dunbar, L.A.; Mac Raighne, A.; Donegan, J.F.; McCabe, E.M., Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination, Proceedings of SPIE - The International Society for Optical Engineering, Opto-Ireland 2005: Photonic Engineering, Opto-Ireland 2005: Photonic Engineering, Dublin, 04 April, Brian W. Bowe, Gerald Byrne, Aidan J. Flanagan, Thomas J. Glynn, Jonathan Magee, Gerard M. O'Connor, Ronan F. O'Dowd, Gerard D. O'Sullivan, John T. Sheridan, 5827, SPIE, 2005, 299 - 304
Three dimensional.pdf (Published (publisher's copy) - Peer Reviewed) 183.3Kb
In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melamine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.
Other Titles:Proceedings of SPIE - The International Society for Optical Engineering, Opto-Ireland 2005: Photonic Engineering
Opto-Ireland 2005: Photonic Engineering
Type of material:Poster
Availability:Full text available