Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination
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2005Citation:
Rakovich, Y.P.; Yang, L.; Taylor, C.M.; Dunbar, L.A.; Mac Raighne, A.; Donegan, J.F.; McCabe, E.M., Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination, Proceedings of SPIE - The International Society for Optical Engineering, Opto-Ireland 2005: Photonic Engineering, Opto-Ireland 2005: Photonic Engineering, Dublin, 04 April, Brian W. Bowe, Gerald Byrne, Aidan J. Flanagan, Thomas J. Glynn, Jonathan Magee, Gerard M. O'Connor, Ronan F. O'Dowd, Gerard D. O'Sullivan, John T. Sheridan, 5827, SPIE, 2005, 299 - 304Download Item:
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Abstract:
In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by
conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a
physical picture on the imaging of the microspheres. Emission spectra from melamine formaldehyde
microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been
experimentally studied. We adopted analytical expressions describing the resonance spacing in order to
determine the size of the microspheres.
Author's Homepage:
http://people.tcd.ie/jdoneganhttp://people.tcd.ie/rakovicy
http://people.tcd.ie/emccabe
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PUBLISHEDDublin
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Proceedings of SPIE - The International Society for Optical Engineering, Opto-Ireland 2005: Photonic EngineeringOpto-Ireland 2005: Photonic Engineering
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