Cyclen-based lanthanide complexes as ribonuclease mimics

Abstract

This thesis, entitled "Novel Cyclen-based Lanthanide Complexes as Ribonuclease Mimics" is divided into six chapters. Chapter One introduces the field of mRNA cleavage, with emphasis on the hydrolysis of phosphodiesters. First discussed are nature's methods of cleaving mRNA and phosphodiesters, concentrating on the use of ribozymes and ribonucleases. The role of metal ions in these reactions is analysed. The use of metal ions to create artificial ribozyme and ribonuclease mimics is discussed, with an emphasis on the use of lanthanides and the importance of using complexed metals. A review of cyclen-based lanthanide complexes that have been used to promote the hydrolysis of phosphodiesters is presented and discussion of selective mRNA cleavage is given.