Automatic Mixing of Bio-Samples Using Micro-Channel and Centrifugation

Abstract

Automatic mixing of bio-samples using micro-channel and centrifugation is considered. Existing methods for mixing bio-samples for life science applications use micro-well and micro-stirrer which are ineffective when used with highly viscous materials at the microliter or nanoliter level. Our method mixes viscous bio-samples in micro-capsules using micro-channel and centrifugation which minimizes contact with mixing tools. To introduce the method, firstly the design of the micro-capsule along with the micro-channel is presented. Secondly, a hydrodynamic model describing the flow of viscous materials in the micro-channel is presented, and the average sample velocity and the traveling time through the micro-channel are analyzed. Thirdly, the relationship between centrifugation speed and time is given to achieve effective and efficient control of the flow. Finally, experimental and theoretical results are compared.