Simplifying and improving the extraction of nitrate from freshwater for stable isotope analyses

Abstract

Determining the isotopic composition of nitrate (NO3 ) in water can prove useful to identify NO3 sources and to understand its dynamics in aquatic systems. Among the procedures available, the `ion-exchange resin method? involves extracting NO3 from freshwater and converting it into solid silver nitrate (AgNO3), which is then analysed for 15N/14N and 18O/16O ratios. This study describes a simplified methodology where water was not pre-treated to remove dissolved organic carbon (DOC) or barium cations (added to precipitate O-bearing contaminants), which suited samples with high NO3 (<400 uM or 25 mgL-1 NO3) and low DOC (typically <417 uM of C or 5 mgL-1 C) levels. % N analysis revealed that a few AgNO3 samples were of low purity (compared with expected % N of 8.2), highlighting the necessity to introduce quality control / quality assurance procedures for silver nitrate prepared from field water samples. Recommendations are then made to monitor % N together with % O (expected at 28.6, i.e. 3.5 fold % N) in AgNO3 in order to better assess the type and gravity of the contamination as well as to identify potentially unreliable data.