Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation
File Type:
PDFItem Type:
Journal ArticleDate:
2021Author:
Access:
openAccessCitation:
Mukhopadhya A, Santoro J, O'Driscoll L, Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation, Star Protocols, 2, 4, 2021, 100821 - 100830Download Item:
STAR.pdf (PDF) 5.825Mb
Abstract:
Separation of highly enriched extracellular vesicles (EVs) fractions from milk is desirable for quantification, cargo analysis, functional characterization, and investigation as delivery vehicles for nutrients and/or therapeutics. However, a rigorous, reproducible protocol is lacking. This protocol considers a crucial aspect typically overlooked, i.e., that caseins are of similar size to, but more abundant than, EVs in milk. Our protocol combines acid pre-treatment and gradient ultracentrifugation, producing EV-enriched fractions suitable for downstream orthogonal characterization approaches.
For complete details on the use and execution of this protocol, please refer to Mukhopadhya et al. (2021).
Author's Homepage:
http://people.tcd.ie/lodrisc
Author: O'Driscoll, Lorraine
Type of material:
Journal ArticleSeries/Report no:
Star Protocols;2;
4;
Availability:
Full text availableKeywords:
Health Sciences, Molecular Biology, Protein Biochemistry, Protein expression and purification, Biotechnology and bioengineeringSubject (TCD):
Cancer , Extracellular Vesicles , Extracellular vesicles (EVs) , exosomesDOI:
10.1016/j.xpro.2021.100821Licences: