Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap.
File Type:
PDFItem Type:
Journal ArticleDate:
2011Citation:
Bazou D, Kearney R, Mansergh F, Bourdon C, Farrar J, Wride M, Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap., Ultrasound in medicine & biology, 37, 2, 2011, 321-30Download Item:
Gene Expression Analysis of Mouse Embryonic Stem Cells Following Levitation in an Ultrasound Standing Wave Trap.pdf (Published (publisher's copy) - Peer Reviewed) 702.0Kb
Abstract:
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a
novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures
(0.08?0.85 MPa) and times (5?60 min) was performed. Our results showed that levitation of ES
cells at the highest employed acoustic pressure for 60 min does not modify gene expression and
cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural
differentiation markers, which were also unaffected by the acoustic field. Our results suggest that
the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES
cell replication and EB differentiation proceed without significantly affecting gene expression.
The technique holds great promise in safe cell manipulation techniques for a variety of
applications including tissue engineering and regenerative medicine.
Sponsor
Grant Number
European Commission
MTKD-CT-2006- 042519 - Nanofab
Health Research Board (HRB)
H01242
Royal Society
2006/R2
Wellcome Trust
089877/Z/09/Z
Author's Homepage:
http://people.tcd.ie/wridemhttp://people.tcd.ie/gjfarrar
Description:
PUBLISHEDType of material:
Journal ArticleSeries/Report no:
Ultrasound in medicine & biology37
2
Availability:
Full text availableKeywords:
Biochemistry, Embryonic stem cellsSubject (TCD):
Genes & Society , Nanoscience & Materials , Next Generation Medical DevicesDOI:
http://dx.doi.org/10.1016/j.ultrasmedbio.2010.10.019ISSN:
0301-5629Licences: