dc.contributor.author | FALLON, PADRAIC GERARD | en |
dc.date.accessioned | 2010-08-20T14:08:58Z | |
dc.date.available | 2010-08-20T14:08:58Z | |
dc.date.issued | 1998 | en |
dc.date.submitted | 1998 | en |
dc.identifier.citation | Pereira, C., Fallon, P.G., Cornette, J., Capron, A., Doenhoff, M.J. & Pierce, R.J., Alterations in cytochrome-c oxidase expression between praziquantel-resistant and susceptible strains of Schistosoma mansoni., Parasitology, 117, 1, 1998, 63 - 73 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/40564 | |
dc.description | PUBLISHED | en |
dc.description | PMID: 9695101 | en |
dc.description.abstract | The genetic differences between praziquantel-resistant (R) and susceptible (S) strains of Schistosoma mansoni (Fallon &
Doenhoff, 1994) were explored using RAPD and by cloning differentially expressed mRNAs by subtractive PCR. No
differences between the 2 strains were detectable by RAPD using 41 different primers indicating that no major genomic
rearrangements were present. Subtractive PCR generated a number of fragments, 1 of which was shown to correspond
to an over-expressed mRNA in the R strain and to encode a fragment of the subunit 1 of cytochrome-c oxidase (SCOX1).
In the absence of a complete sequence for this gene, we used EST sequences to compile a consensus sequence for the
904 bp at the 3< end that enabled us to choose primers for semi-quantitative RT?PCR. This technique showed that SCOX1
was indeed over-expressed about 5 to 10-fold in the R strain whereas the genes encoding the 28 kDa glutathione Stransferase,
glutathione peroxidase, NADH dehydrogenase subunit 5 and the ATP-binding cassette family protein
SMDR2 were not. In contrast, cytochrome-c oxidase enzyme activity was 4-fold lower in the R strain than in the S strain. | en |
dc.description.sponsorship | This work was supported by INSERM and the CNRS,
and by the UNDP}World Bank}WHO Special Program
for Research and Training in Tropical Diseases, the
Commission of the European Communities under the
Program for Science and Technology for Development
and the Wellcome Trust (London, UK). C.P. received
?nancial support from the CAPES (Brasil) and from the
Institut Pasteur, Lille. We thank Monique Leroux and
Suzanne Van Wingene for technical assistance and Volvic
S.A. for support for the maintenance of the parasite lifecycle. | en |
dc.format.extent | 63 | en |
dc.format.extent | 73 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Parasitology | en |
dc.relation.ispartofseries | 117 | en |
dc.relation.ispartofseries | 1 | en |
dc.rights | Y | en |
dc.subject | Immunology | en |
dc.subject | Schistosoma mansoni | en |
dc.title | Alterations in cytochrome-c oxidase expression between praziquantel-resistant and susceptible strains of Schistosoma mansoni. | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/pfallon | en |
dc.identifier.rssinternalid | 25503 | en |
dc.identifier.rssuri | http://dx.doi.org/10.1017/S003118209800273X | en |
dc.contributor.sponsor | European Union (EU) | en |