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dc.contributor.authorFALLON, PADRAIC GERARDen
dc.date.accessioned2010-08-20T14:08:58Z
dc.date.available2010-08-20T14:08:58Z
dc.date.issued1998en
dc.date.submitted1998en
dc.identifier.citationPereira, C., Fallon, P.G., Cornette, J., Capron, A., Doenhoff, M.J. & Pierce, R.J., Alterations in cytochrome-c oxidase expression between praziquantel-resistant and susceptible strains of Schistosoma mansoni., Parasitology, 117, 1, 1998, 63 - 73en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/40564
dc.descriptionPUBLISHEDen
dc.descriptionPMID: 9695101en
dc.description.abstractThe genetic differences between praziquantel-resistant (R) and susceptible (S) strains of Schistosoma mansoni (Fallon & Doenhoff, 1994) were explored using RAPD and by cloning differentially expressed mRNAs by subtractive PCR. No differences between the 2 strains were detectable by RAPD using 41 different primers indicating that no major genomic rearrangements were present. Subtractive PCR generated a number of fragments, 1 of which was shown to correspond to an over-expressed mRNA in the R strain and to encode a fragment of the subunit 1 of cytochrome-c oxidase (SCOX1). In the absence of a complete sequence for this gene, we used EST sequences to compile a consensus sequence for the 904 bp at the 3< end that enabled us to choose primers for semi-quantitative RT?PCR. This technique showed that SCOX1 was indeed over-expressed about 5 to 10-fold in the R strain whereas the genes encoding the 28 kDa glutathione Stransferase, glutathione peroxidase, NADH dehydrogenase subunit 5 and the ATP-binding cassette family protein SMDR2 were not. In contrast, cytochrome-c oxidase enzyme activity was 4-fold lower in the R strain than in the S strain.en
dc.description.sponsorshipThis work was supported by INSERM and the CNRS, and by the UNDP}World Bank}WHO Special Program for Research and Training in Tropical Diseases, the Commission of the European Communities under the Program for Science and Technology for Development and the Wellcome Trust (London, UK). C.P. received ?nancial support from the CAPES (Brasil) and from the Institut Pasteur, Lille. We thank Monique Leroux and Suzanne Van Wingene for technical assistance and Volvic S.A. for support for the maintenance of the parasite lifecycle.en
dc.format.extent63en
dc.format.extent73en
dc.language.isoenen
dc.relation.ispartofseriesParasitologyen
dc.relation.ispartofseries117en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectImmunologyen
dc.subjectSchistosoma mansonien
dc.titleAlterations in cytochrome-c oxidase expression between praziquantel-resistant and susceptible strains of Schistosoma mansoni.en
dc.typeJournal Articleen
dc.contributor.sponsorEuropean Union (EU)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pfallonen
dc.identifier.rssinternalid25503en
dc.identifier.rssurihttp://dx.doi.org/10.1017/S003118209800273Xen


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