Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells.
Citation:
Hennessy E, Clynes M, Jeppesen P, O'Driscoll L, Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells., Biochemical and Biophysical Research Communications, 396, 2, 2010, 457 - 462Abstract:
MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density human microRNA arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homolog.
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Grant Number
Health Research Board (HRB)
Author's Homepage:
http://people.tcd.ie/lodriscDescription:
PUBLISHED
Author: O'DRISCOLL, LORRAINE
Type of material:
Journal ArticleSeries/Report no:
Biochemical and Biophysical Research Communications396
2
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Full text availableKeywords:
microRNA, GSIS, insulin secretion, MIN6Subject (TCD):
Genes & SocietyDOI:
http://dx.doi.org/10.1016/j.bbrc.2010.04.116Metadata
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