Development of SIRT1 siRNA-carrying liposomes for treatment of prostate cancer
Citation:
Debelec-Butuner B, Oner E, Kotmakci M, Kantarci AG, Development of SIRT1 siRNA-carrying liposomes for treatment of prostate cancer, BioTurkey International Biotechnology Congress, Istanbul, Turkey, 5-7 March 2020, 2020Download Item:
Abstract:
Sirtuin 1 (SIRT1) is a histone deacetylase (HDAC) from Sirtuin family and high
expression of it induces activation of DNA repairing factors eventually leading either
tumor suppression or tumor development in cellular context-dependent manner.
Since most of the chemotherapeutics induce apoptosis by creating DNA damage in
tumor cells and further SIRT1-activated repair of the chemotherapeutic-induced
DNA damage results in decreased apoptosis, inhibition of SIRT1 is a promising
strategy both in anticancer therapy and preventing chemotherapy resistance. High
expression level of SIRT1 in prostate cancer level has been also reported in many
cases and it has been confirmed in our context by showing the higher protein levels
of SIRT1 in prostate cancer cell lines than normal prostate epithelium cells. As, gene
silencing of oncogenes via RNA interference technology is a recent approach in
cancer therapy, in this study, siRNA carrying liposomal formulations composed of
N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) as
cationic lipid have been formulated by freeze drying method for SIRT1 inhibition.
In addition to characterization of the liposomes, their efficiency on SIRT1 silencing
has been shown both on mRNA and protein levels on prostate cancer cell lines
LNCaP, Du145, and PC-3. It has also been determined that each liposomal
formulation has different levels of efficiency on each cell line so it has been achieved
to develop an appropriate nucleic acid delivery formulation for SIRT1 silencing for
different metastatic prostate cancer types.
Author's Homepage:
http://people.tcd.ie/onere
Author: Oner-Bozkurt, Ezgi
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BioTurkey International Biotechnology CongressCollections
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Full text availableKeywords:
Liposome, SIRT1, nucleic acid delivery, prostate cancerSubject (TCD):
Cancer , Nanoscience & Materials , Drug Delivery Systems , Gene therapy , Nano Biotechnology , NanoMedicine , Nanotechnology , Prostate cancerMetadata
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