Physiological activation of mGlu5 receptors supports the ion channel function of NMDA receptors in hippocampal LTD induction in vivo

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Journal ArticleDate:
2018Access:
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O'Riordan KJ, Hu NW, Rowan MJ, Physiological activation of mGlu5 receptors supports the ion channel function of NMDA receptors in hippocampal LTD induction in vivo, Scientific Reports, 8, 1, 2018, 4391Download Item:
Abstract:
Synaptic long-term depression (LTD) is believed to underlie critical mnemonic processes in the adult hippocampus. The roles of the metabotropic and ionotropic actions of glutamate in the induction of synaptic LTD by electrical low-frequency stimulation (LFS) in the living adult animal is poorly understood. Here we examined the requirement for metabotropic glutamate (mGlu) and NMDA receptors in LTD induction in anaesthetized adult rats. LTD induction was primarily dependent on NMDA receptors and required the involvement of both the ion channel function and GluN2B subunit of the receptor. Endogenous mGlu5 receptor activation necessitated the local application of relatively high doses of either competitive or non-competitive NMDA receptor antagonists to block LTD induction. Moreover, boosting endogenous glutamate activation of mGlu5 receptors with a positive allosteric modulator lowered the threshold for NMDA receptor-dependent LTD induction by weak LFS. The present data provide support in the living animal that NMDA receptor-dependent LTD is boosted by endogenously released glutamate activation of mGlu5 receptors. Given the predominant perisynaptic location of mGlu5 receptors, the present findings emphasize the need to further evaluate the contribution and mechanisms of these receptors in NMDA receptor-dependent synaptic plasticity in the adult hippocampus in vivo.
Sponsor
Grant Number
Health Research Board (HRB)
HRA-POR-2015-1102
National Natural Science Foundation of China (60703033).
81471114
Science Foundation Ireland (SFI)
14/IA/2571
Science Foundation Ireland (SFI)
10/IN.1/B3001
Author's Homepage:
http://people.tcd.ie/mrowanhttp://people.tcd.ie/hunw
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PUBLISHED
Author: Rowan, Michael; Hu, Neng
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Journal ArticleSeries/Report no:
Scientific Reports8
1
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Neuroscience , Molecular BiologyDOI:
http://dx.doi.org/10.1038/s41598-018-22768-xMetadata
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