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dc.contributor.authorRADOMSKI, MAREKen
dc.contributor.authorSANTOS-MARTINEZ, MARIAen
dc.date.accessioned2016-01-07T15:45:15Z
dc.date.available2016-01-07T15:45:15Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationInkielewicz-Stepniak, I., Santos-Martinez, M.J., Medina, C., Radomski, M.W., Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride, International Journal of Nanomedicine, 9, 2014, 1677 - 1687en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/75511
dc.descriptionPUBLISHEDen
dc.description.abstractBACKGROUND: Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine and dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells and tissues to these xenobiotics are not clear. Therefore, we have evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast cells. METHODS: Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs was examined by transmission electron microscopy. Downstream inflammatory effects and oxidative stress were measured by real-time quantitative polymerase chain reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and real-time quantitative PCR and flow cytometry, respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK) was studied using Western blot. RESULTS: We found that AgNPs penetrated the cell membrane and localized inside the mitochondria. Co-incubation experiments resulted in increased oxidative stress, inflammation, and apoptosis. In addition, we found that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK. CONCLUSION: A combined exposure of human fibroblasts to AgNPs and F results in increased cellular damage. Further studies are needed in order to evaluate pharmacological and potentially toxicological effects of AgNPs and F on oral health.en
dc.format.extent1677en
dc.format.extent1687en
dc.language.isoenen
dc.relation.ispartofseriesInternational Journal of Nanomedicineen
dc.relation.ispartofseries9en
dc.rightsYen
dc.subjectoxidative stressen
dc.titlePharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluorideen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/radomskmen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/santosmmen
dc.identifier.rssinternalid97690en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeNanoscience & Materialsen
dc.subject.TCDTagNanopharmacologyen


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