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dc.contributor.authorFOSTER, TIMOTHY JAMES
dc.date.accessioned2010-04-27T14:12:17Z
dc.date.available2010-04-27T14:12:17Z
dc.date.issued2010
dc.date.submitted2010en
dc.identifier.citationBarbu, EM, Ganesh, VK, Gurusiddappa, S, MacKenzie, RC, Foster, TJ, Sudhof, TC, Hook, M, Beta-neurexin is a ligand for the Staphylococcus aureus MSCRAMM SdrC, PloS Pathogens, 6, 1, 2010, e1000726-en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/39218
dc.descriptionPUBLISHEDen
dc.description.abstractGram-positive bacteria contain a family of surface proteins that are covalently anchored to the cell wall of the organism. These cell-wall anchored (CWA) proteins appear to play key roles in the interactions between pathogenic organisms and the host. A subfamily of the CWA has a common structural organization with multiple domains adopting characteristic IgG-like folds. The identified microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) belong to this subfamily, as does SdrC from S. aureus. However, an interactive host ligand for the putative MSCRAMM SdrC was not previously identified. We have screened a phage display peptide library and identified a peptide sequence found in beta-neurexin that binds SdrC. A synthetic peptide corresponding to the identified sequence as well as a recombinant form of the beta-neurexin 1 exodomain binds SdrC with high affinity and specificity. Furthermore, expression of SdrC on bacteria greatly enhances microbial adherence to cultured mammalian cells expressing beta-neurexin on their surface. Taken together, our experimental results demonstrate that beta-neurexin is a ligand for SdrC. This interaction involves a specific sequence located in the N-terminal region of the mammalian protein and the N(2)N(3) domain of the MSCRAMM. The fact that these two proteins interact when expressed on the appropriate cells demonstrates the functionality of the interaction. Possible implications of this interaction are discussed.en
dc.description.sponsorshipFunding source: NIH/NIAMS AI20624-22 ``Molecular Analysis of Staphylococcus aureus Host Interactions?? (http://www.niams.nih.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.format.extente1000726en
dc.language.isoenen
dc.relation.ispartofseriesPloS Pathogens;
dc.relation.ispartofseries6;
dc.relation.ispartofseries1;
dc.rightsYen
dc.subjectMicrobiology
dc.titleBeta-neurexin is a ligand for the Staphylococcus aureus MSCRAMM SdrCen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/tfoster
dc.identifier.rssinternalid63962


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