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dc.contributor.advisorMcLoughlin, Rachel
dc.contributor.authorCooper, Andrew
dc.date.accessioned2021-10-04T10:57:14Z
dc.date.available2021-10-04T10:57:14Z
dc.date.issued2021en
dc.date.submitted2021
dc.identifier.citationCooper, Andrew, The activation of unconventional T cells by Staphylococcus aureus, Trinity College Dublin.School of Biochemistry & Immunology, 2021en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/97216
dc.descriptionAPPROVEDen
dc.description.abstractStaphylococcus aureus is a major human pathogen of multiple tissue sites. The emergence of antibiotic-resistant strains promises greater morbidity in the future. No S. aureus vaccine has so far succeeded in translating murine immunity to human patients. Unconventional T cells may be crucial for responses to S. aureus. Murine γδ T cells mediate immunity to S. aureus, and MAIT cells respond to S. aureus antigens in vitro. However, the mechanics of these responses remain unclear. Here I have investigated the potential for S. aureus-induced activation of human γδ T and MAIT cells to aid in the development of the S. aureus vaccine. I have found that while γδ T cells respond rapidly and robustly in the mouse, these responses were tissue-specific. I have also found a number of clear disparities between γδ T cell responses of mice and humans, helping to explain repeated failed vaccines. In co-culture with infected DCs, I have found that Vδ2+ cells secrete high levels of IFNγ, a response initiated by DC contact and enhanced by IL-12. In return, γδ T cells upregulate DC activation in a positive feedback loop. I have also found that activation of CD4+ T cells in co- culture is also enhanced by γδ T cells. I have also investigated the potential for S. aureus to activate MAIT cells, and found that after binding of DC-expressed MR1, MAIT cells commenced secretion of both IFNγ and cytotoxic mediators. My findings implicate these unconventional T cell subsets in important, rapid anti-S. aureus responses that may be of great relevance to the ongoing development of novel anti-S. aureus treatments.en
dc.language.isoenen
dc.publisherTrinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistryen
dc.rightsYen
dc.subjectImmunologyen
dc.subjectMRSAen
dc.subjectStaphylococcusen
dc.subjectaureusen
dc.subjectgd T cellsen
dc.subjectMAIT cellsen
dc.titleThe activation of unconventional T cells by Staphylococcus aureusen
dc.typeThesisen
dc.type.supercollectionthesis_dissertationsen
dc.type.supercollectionrefereed_publicationsen
dc.type.qualificationlevelDoctoralen
dc.identifier.peoplefinderurlhttps://tcdlocalportal.tcd.ie/pls/EnterApex/f?p=800:71:0::::P71_USERNAME:COOPERANen
dc.identifier.rssinternalid233891en
dc.rights.ecaccessrightsembargoedAccess
dc.date.ecembargoEndDate2025-01-01
dc.contributor.sponsorTCDen
dc.contributor.sponsorScience Foundation Ireland (SFI)en


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