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dc.contributor.authorO'Driscoll, Lorraine
dc.contributor.authorThéry, Clotilde
dc.contributor.authorWitwer, Kenneth W.
dc.contributor.authorAikawa, Elena
dc.contributor.authorAlcarez, Maria Jose
dc.contributor.authorAnderson, Johnathon D.
dc.contributor.authorAndriantsitohaina, Ramaroson
dc.contributor.authorAntoniou, Anna
dc.contributor.authorArab, Tanina
dc.contributor.authorArcher, Fabienne
dc.contributor.authorAtkin-Smith, Georgia K.
dc.contributor.authorAyre, D. Craig
dc.contributor.authorBach, Jean-Marie
dc.contributor.authorBachurski, Daniel
dc.contributor.authorBaharvand, Hossein
dc.contributor.authorBalaj, Leonora
dc.contributor.authorBaldacchino, Shawn
dc.contributor.authorBauer, Natalie N.
dc.contributor.authorBaxter, Amy A.
dc.contributor.authorBebawy, Mary
dc.contributor.authorBeckham, Carla
dc.contributor.authorBedina Zavec, Apolonija
dc.contributor.authorBenmoussa, Abderrahim
dc.contributor.authorBerardi, Anna C.
dc.contributor.authorBergese, Paolo
dc.contributor.authorBielska, Ewa
dc.contributor.authorBlenkiron, Cherie
dc.contributor.authorBobis-Wozowicz, Sylwia
dc.contributor.authorBoilard, Eric
dc.contributor.authorBoireau, Wilfrid
dc.date.accessioned2019-12-13T12:05:27Z
dc.date.available2019-12-13T12:05:27Z
dc.date.issued2019
dc.date.submitted2019en
dc.identifier.citationThery C, Wiwer, K.W., O'Driscoll L, et al., Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines, J. Extracell. Vesicles, 2019, 1--en
dc.identifier.otherY
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/20013078.2018.1535750
dc.identifier.urihttp://hdl.handle.net/2262/91089
dc.description.abstractThe last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.en
dc.format.extent1-en
dc.language.isoenen
dc.relation.ispartofseriesJ. Extracell. Vesicles;
dc.rightsYen
dc.subjectExtracellular vesiclesen
dc.subjectExosomesen
dc.subjectEctosomesen
dc.subjectMicrovesiclesen
dc.subjectMinimal informationen
dc.subjectRequirementsen
dc.subjectGuidelinesen
dc.subjectStandardizationen
dc.subjectMicroparticlesen
dc.subjectRigoren
dc.subjectReproducibilityen
dc.titleMinimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelinesen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/lodrisc
dc.identifier.rssinternalid195091
dc.identifier.doihttps://doi.org/10.1080/20013078.2018.1535750
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeCanceren
dc.subject.TCDTagExtracellular Vesiclesen
dc.subject.TCDTagExtracellular vesicles (EVs)en
dc.identifier.rssurihttps://www.tandfonline.com/doi/full/10.1080/20013078.2018.1535750
dc.identifier.orcid_id0000-0002-9860-8262
dc.status.accessibleNen


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