Structural and functional studies of Pseudomonas aeruginosa strain PAOI Lysine Specific Permease (LysP)

Abstract

The means by which substrates are shuttled across prokaryotic membranes by membrane transporters and the conformational changes involved are unique to each transporter. To decipher the underlying mechanism of substrate selectivity and the conformational changes required for transport, we sought to establish the high resolution crystal structure of a lysine specific transporter from Pseudomonas aeruginosa strain PAOl, hereafter referred to as LysP. Also, we aim to show that the protein binds and transports L-lysine.