The molecular chaperone dnak is a source of mutational robustness
Citation:
Aguilar-RodrÃguez J, Sabater-Muñoz B, Montagud-MartÃnez R, Berlanga V, Alvarez-Ponce D, Wagner A, Fares M.A, The molecular chaperone dnak is a source of mutational robustness, Genome Biology and Evolution, 8, 9, 2016, 2979 - 2991Download Item:
Abstract:
Molecular chaperones, also known as heat-shock proteins, refold misfolded proteins and help other proteins reach their native conformation. Thanks to these abilities, some chaperones, such as the Hsp90 protein or the chaperonin GroEL, can buffer the deleterious phenotypic effects of mutations that alter protein structure and function. Hsp70 chaperones use a chaperoning mechanism different from that of Hsp90 and GroEL, and it is not known whether they can also buffer mutations. Here, we show that they can. To this end, we performed a mutation accumulation experiment in Escherichia coli, followed by whole-genome resequencing. Overexpression of the Hsp70 chaperone DnaK helps cells cope with mutational load and completely avoid the extinctions we observe in lineages evolving without chaperone overproduction. Additionally, our sequence data show that DnaK overexpression increases mutational robustness, the tolerance of its clients to nonsynonymous nucleotide substitutions. We also show that this elevated mutational buffering translates into differences in evolutionary rates on intermediate and long evolutionary time scales. Specifically, we studied the evolutionary rates of DnaK clients using the genomes of E. coli, Salmonella enterica, and 83 other gamma-proteobacteria. We find that clients that interact strongly with DnaK evolve faster than weakly interacting clients. Our results imply that all three major chaperone classes can buffer mutations and affect protein evolution. They illustrate how an individual protein like a chaperone can have a disproportionate effect on the evolution of a proteome.
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Grant Number
Science Foundation Ireland (SFI)
12/IP/1673
Author's Homepage:
http://people.tcd.ie/faresmDescription:
PUBLISHEDCited By :2 Export Date: 23 March 2017
Author: FARES, MARIO
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Journal ArticleCollections
Series/Report no:
Genome Biology and Evolution8
9
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Molecular chaperoneDOI:
http://dx.doi.org/10.1093/gbe/evw176Metadata
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