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dc.contributor.advisorO'Neill, Luke
dc.contributor.authorBecker, Christine
dc.date.accessioned2017-01-18T10:14:50Z
dc.date.available2017-01-18T10:14:50Z
dc.date.issued2010
dc.identifier.citationChristine Becker, 'An investigation into novel components and signalling mechanisms of inflammasomes', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Medicine, 2010, pp 305
dc.identifier.otherTHESIS 9463
dc.identifier.urihttp://hdl.handle.net/2262/78812
dc.description.abstractThis thesis concerns an analysis of caspase-1 and ASC, key components of the inflammasome. At the outset Rab39a had been found in a complex with caspase-1. Rab39a is a member of the Rab GTPase family, a group of proteins which have important roles in protein trafficking and secretion. Rab39a was successfully cloned and its binding to caspase-1 was confirmed by co-immunoprecipitation and confocal microscopy experiments. Biological assays were carried out to try and determine the ftinctional relevance of this interaction. Bioinformatic analysis showed that Rab39a contains a highly conserved caspase-1 cleavage site and can be cleaved in the presence of recombinant caspase-1 or in cells treated with LPS and ATP. Rab39a was found to localise with active caspases on phagosomes formed in response to S. aureus and was shown to have a role in bacterial uptake. The Rab39a promoter contains many putative transcription factor binding sites and Real Time PCR analysis showed that Rab39a mRNA levels are significantly increased in response to LPS, Malp2 and Pam3Cys.
dc.format1 volume
dc.language.isoen
dc.publisherTrinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Medicine
dc.relation.isversionofhttp://stella.catalogue.tcd.ie/iii/encore/record/C__Rb14882918
dc.subjectBiochemistry and Immunology, Ph.D.
dc.subjectPh.D. Trinity College Dublin
dc.titleAn investigation into novel components and signalling mechanisms of inflammasomes
dc.typethesis
dc.type.supercollectionthesis_dissertations
dc.type.supercollectionrefereed_publications
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (Ph.D.)
dc.rights.ecaccessrightsopenAccess
dc.format.extentpaginationpp 305
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