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dc.contributor.advisorClaffey, Noel
dc.contributor.authorAlhag, Mohamed
dc.date.accessioned2016-11-24T16:54:10Z
dc.date.available2016-11-24T16:54:10Z
dc.date.issued2009
dc.identifier.citationMohamed Alhag, 'Collagen-glycosaminoglycan scaffold and mesenchymal stem cells for bone tissue engineering', [thesis], Trinity College (Dublin, Ireland). School of Dental Science, 2009, pp 216
dc.identifier.otherTHESIS 9401
dc.identifier.urihttp://hdl.handle.net/2262/77849
dc.description.abstractTissue engineering using cell-seeded biodegradable scaffolds offers a new bone regenerative approach that might circumvent many of the limitations of current therapeutic modalities. This thesis reports on a number of in vitro and in vivo aspects of bone tissue engineering using collagen-glycosaminoglycan scaffolds and bone marrow derived, murine mesenchymal stem cells. The work presented in chapter 3 quantified the dimensional changes as a result of cell- mediated contraction in mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffolds when cultured in osteoinductive factor-supplemented medium. The effect of cells originating from different donors on the scaffold contraction was also examined. Three experimental groups were used; non-seeded scaffolds (Group 1), seeded scaffolds which were maintained in standard culture medium for 4 weeks (Group 2), and seeded scaffolds which were maintained in osteoinductive factor-supplemented medium for 4 weeks (Group 3) i.e. a tissue engineered group which allowed matrix deposition and mineralisation upon the scaffolds prior to implantation. Each group comprised 3 scaffolds of each of the following diameters (6 mm, 10 mm, 13 mm, and 16 mm) for a total of 12 scaffolds in each group. After 4 weeks in culture, seeded scaffolds displayed significantly greater contraction than the cell-free scaffolds (P < 0.0001). However, the difference between the percentage reduction in diameter of scaffolds in group 2 (49.11 ± 2.36 %) and in group 3 (44.26 ± 2.68 %) was not significant. Scaffolds with larger diameters showed substantially more contraction than scaffolds with smaller diameters (P < 0.001). Similar amounts of contraction were reached when the scaffolds were seeded with cells originating from different donors (P < 0.32).
dc.format1 volume
dc.language.isoen
dc.publisherTrinity College (Dublin, Ireland). School of Dental Science
dc.relation.isversionofhttp://stella.catalogue.tcd.ie/iii/encore/record/C__Rb14880829
dc.subjectDental Science, Ph.D.
dc.subjectPh.D. Trinity College Dublin
dc.titleCollagen-glycosaminoglycan scaffold and mesenchymal stem cells for bone tissue engineering
dc.typethesis
dc.type.supercollectionthesis_dissertations
dc.type.supercollectionrefereed_publications
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (Ph.D.)
dc.rights.ecaccessrightsopenAccess
dc.format.extentpaginationpp 216
dc.description.noteTARA (Trinity’s Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.ie


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