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dc.contributor.authorCAFFREY, MARTINen
dc.date.accessioned2016-01-08T14:41:19Z
dc.date.available2016-01-08T14:41:19Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationWeierstall, U., James, D., Wang, C., (...), Caffrey, M., Cherezov, V., Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography, Nature Communications, 5, 2014, 3309-en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/75530
dc.descriptionPUBLISHEDen
dc.description.abstractLipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.en
dc.format.extent3309en
dc.relation.ispartofseriesNature Communicationsen
dc.relation.ispartofseries5en
dc.rightsYen
dc.subjectLipidic cubic phase (LCP)en
dc.titleLipidic cubic phase injector facilitates membrane protein serial femtosecond crystallographyen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcaffreen
dc.identifier.rssinternalid94982en
dc.identifier.doihttp://dx.doi.org/10.1038/ncomms4309en
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorNational Science Foundation (NSF)en
dc.contributor.sponsorGrantNumberMCB 1120997en
dc.contributor.sponsorNational Science Foundation (NSF)en
dc.contributor.sponsorGrantNumberMCB 1021557en


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