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dc.contributor.authorCAFFREY, MARTINen
dc.date.accessioned2015-06-02T15:27:27Z
dc.date.available2015-06-02T15:27:27Z
dc.date.issued2014en
dc.date.submitted2014en
dc.identifier.citationCaffrey, M. Eifert, R. Li, D. Howe, N., The lipid cubic phase or in meso method for crystallizing proteins. Bushings for better manual dispensing, Journal of Applied Crystallography, 47, 5, 2014, 1804 - 1806en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/74008
dc.descriptionPUBLISHEDen
dc.description.abstractThe lipid cubic phase or in meso method can be used to set up crystallization trials of soluble and membrane proteins. The cubic phase is noted for being a sticky and viscous mesophase. Dispensing the protein-laden mesophase by hand into wells on crystallization plates has been facilitated by the use of an inexpensive repeat dispenser. However, the assembled dispensing device is prone to damage. Specifically, the delicate plunger used to dispense the viscous mesophase by positive displacement can be bent and scarred when the locking nut that fixes the plunger to the ratchet-driven dispensing mechanism is inadvertently overtightened. A defective plunger can render the device useless as a dispensing tool. More importantly, it can lead to catastrophic loss of valuable protein and lipid due to leakage when the dispensing syringe is being recharged with fresh mesophase. This note describes two types of bushings designed to protect the plunger from mechanical damage, which provide facile and reliable dispenser performance. One is a split bushing in brass and is a highly durable solution. The other is a small sleeve made from readily available plastic tubing. While it lacks durability, the plastic sleeve is simple yet highly effective, and can be replaced as the need arisesen
dc.description.sponsorshipThis work was supported in part by grants from Science Founda- tion Ireland (12/IA/1255) and the National Institutes of Health (GM75915, P50GM073210 and U54GM094599)en
dc.format.extent1804en
dc.format.extent1806en
dc.relation.ispartofseriesJournal of Applied Crystallographyen
dc.relation.ispartofseries47en
dc.relation.ispartofseries5en
dc.rightsYen
dc.subjectlipid cubic phaseen
dc.subject.lcshlipid cubic phaseen
dc.titleThe lipid cubic phase or in meso method for crystallizing proteins. Bushings for better manual dispensingen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcaffreen
dc.identifier.rssinternalid102687en
dc.identifier.doihttp://dx.doi.org/10.1107/S1600576714015702en
dc.rights.ecaccessrightsopenAccess
dc.identifier.rssurihttp://www.scopus.com/inward/record.url?eid=2-s2.0-84907486687&partnerID=40&md5=9179cfdafbc5b45cac1ca094cb87bf88en
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.contributor.sponsorGrantNumber12/IA/1255en


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