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dc.contributor.authorCAFFREY, MARTIN
dc.date.accessioned2014-06-26T11:28:30Z
dc.date.available2014-06-26T11:28:30Z
dc.date.issued2010
dc.date.submitted2010en
dc.identifier.citationHöfer N, Aragão D, Caffrey M, Crystallizing membrane proteins for structure determination using lipidic mesophases. , Journal of Visualized Experiments, 45, 2010, 1712-en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/69873
dc.descriptionPUBLISHEDen
dc.description.abstractA detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be quite versatile in that it has been used to solve X-ray crystallographic structures of prokaryotic and eukaryotic proteins, proteins that are monomeric, homo- and hetero-multimeric, chromophore-containing and chromophore-free, and alpha-helical and beta-barrel proteins. Recent successes using in meso crystallization are the human engineered beta2-adrenergic and adenosine A2a G protein-coupled receptors. Protocols are presented for reconstituting the membrane protein into the monoolein-based mesophase, and for setting up crystallizations in the manual mode. Additional steps in the overall process, such as crystal harvesting, are to be addressed in future video articles. The time required to prepare the protein-loaded mesophase and to set up a crystallization plate manually is about one hour.en
dc.format.extent1712en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Visualized Experiments;
dc.relation.ispartofseries45;
dc.rightsYen
dc.subjectcrystallizing membrane proteinsen
dc.subjectProtocolen
dc.titleCrystallizing membrane proteins for structure determination using lipidic mesophases.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcaffre
dc.identifier.rssinternalid68994
dc.rights.ecaccessrightsopenAccess
dc.contributor.sponsorScience Foundation Irelanden


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