Browsing School of Genetics & Microbiology by Date of Publication
Now showing items 1-20 of 896
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Recombination and complementation between R factors in Escherichia coli K12
(1971)Recombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated in Escherichia coli K12 rec+; it occurs at reduced frequency in recB and recC, and is not detectable in reeA, indicating ... -
Deletion map of the chloramphenicol resistance region of R1 and R100-1
(1973)Recombination between single-site and multisite chloramphenicol-sensitive mutants of the F-like R factors R1 and R100-1 indicates that the chloramphenicol resistance region is a single structural gene coding for the ... -
Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K12 chromosome
(1975)Pairs of normally incompatible derivatives of R100-1 (one ChlS TetR, the other ChilR TetS) were forced to coexist in a recA host by selection for ChlR TetR cells. After many generations stable derivatives were isolated. ... -
R factor tetracycline and chloramphenicol resistance in Escherichia coli K12 cmlB mutants
(1975)The isolation of Escherichia coli chromosomal mutants that increased the level of resistance of a partially tetracycline-sensitive mutant of RI00-I is described. Plasmid-less derivatives of these moderately resistant mutants ... -
Deletions in the r-determinant mer region of plasmid R100-1 selected by loss of mercury hypersensitivity
(1979)A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to ... -
Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1
(1979)A series of 23 transposon 801(Tn801)-induced mutations of plasmid R100-1 from mercuric salts resistance to sensitivity was studied. Although Tn801 transposed frequently into the mer region of the plasmid, fine structural ... -
Analysis of tetracycline resistance encoded by transposon Tn10: deletion mapping of tetracycline-sensitive point mutations and identification of two structural genes
(1983)Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. ... -
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100
(1983)The mercuric resistance (mer) genes of plasmid R100 were cloned into plasmid pBR322. A series of transposon Tn5 insertion mutations in the mer genes were isolated and mapped. The mutants were characterized phenotypically ... -
Expression of a cloned Staphylococcus aureus alpha-hemolysin determinant in Bacillus subtilis and Staphylococcus aureus
(1983)A DNA sequence encoding Staphylococcus aureus alpha-hemolysin, which had been previously cloned and mapped in Escherichia coli K-12, was introduced into Bacillus subtilis BD170 and several strains of S. aureus by using ... -
Analysis of the chloramphenicol resistance determinant of plasmid R26
(Trinity College (Dublin, Ireland). Department of Microbiology, 1985)The Inc P-1 plasmid, R2B carries an inducible chloramphenicol resistance determinant, cml. This specifies low level chloramphenicol resistance (30 μg/ml] in Escherichia coli. The mechanism does not involve drug detoxification ... -
Some mercurial resistance plasmids from different incompatibility groups specify merR regulatory functions that both repress and induce the mer operon of plasmid R100
(1985)Transcription of the mer genes of plasmid R100 is regulated by the product of the merR gene. The merR gene negatively regulates its own expression and also controls the transcription of the merTCA operon both negatively ... -
Posttranscriptional regulation of the inducible nonenzymatic chloramphenicol resistance determinant of IncP plasmid R26
(1985)The inducible nonenzymatic chloramphenicol resistance (Cmr) determinant of the IncP plasmid R26 was cloned on a 1,900-base-pair restriction endonuclease HindIII fragment. Transposon Tn5 mutagenesis revealed that at least ... -
Identification of the merR gene of R100 by using mer-lac gene and operon fusions
(1985)Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were ... -
Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis.
(1987)The sensitivity of Bacillus subtilis to hydrogen peroxide (oxidative stress) was found to vary with the position of the culture in the growth cycle. The most dramatic change occurred at the stationary phase, when the cells ... -
Oxidative stress and growth temperature in Bacillus subtilis.
(1987)Pretreatment of Bacillus subtilis with low concentrations of hydrogen peroxide protected the cells against the lethal effects of higher levels of oxidative stress. During the period of adaptation, eight proteins were ... -
Genetic analysis of gentamicin resistance in methicillin- and gentamicin-resistant strains of Staphylococcus aureus isolated in Dublin hospitals.
(American Society for MIcrobiology, 1988)Methicillin- and gentamicin-resistant strains of Staphylococcus aureus isolated in Dublin hospitals have been classified into groups I, II, and III based on resistance to antimicrobial agents and plasmid profiles. Each ... -
CODON USAGE AND GENE-EXPRESSION LEVEL IN DICTYOSTELIUM-DISCOIDEUM - HIGHLY EXPRESSED GENES DO PREFER OPTIMAL CODONS
(1989)Codon usage patterns in the slime mould Dictyostelium discoideum have been re-examined (a total of 58 genes have been analysed). Considering the extreme A + T-richness of this genome (G + C = 22%), there is a surprising ... -
Replication and segregational stability of Bacillus plasmid pBAA1.
(1989)A cryptic plasmid, pBAA1, was identified in an industrial Bacillus strain. The plasmid is 6.8 kilobases in size and is present in cells at a copy number of approximately 5 per chromosome equivalent. The plasmid has been ... -
Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor
(1992)Staphylococcus aureus Newman cells carry a surface receptor for fibrinogen called clumping factor. The bacteria also express coagulase, an extracellular protein that binds to prothrombin to form a complex with thrombinlike ...
