Browsing Microbiology (Scholarly Publications) by Date of Publication
Now showing items 1-20 of 237
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Recombination and complementation between R factors in Escherichia coli K12
(1971)Recombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated in Escherichia coli K12 rec+; it occurs at reduced frequency in recB and recC, and is not detectable in reeA, indicating ... -
Deletion map of the chloramphenicol resistance region of R1 and R100-1
(1973)Recombination between single-site and multisite chloramphenicol-sensitive mutants of the F-like R factors R1 and R100-1 indicates that the chloramphenicol resistance region is a single structural gene coding for the ... -
Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K12 chromosome
(1975)Pairs of normally incompatible derivatives of R100-1 (one ChlS TetR, the other ChilR TetS) were forced to coexist in a recA host by selection for ChlR TetR cells. After many generations stable derivatives were isolated. ... -
R factor tetracycline and chloramphenicol resistance in Escherichia coli K12 cmlB mutants
(1975)The isolation of Escherichia coli chromosomal mutants that increased the level of resistance of a partially tetracycline-sensitive mutant of RI00-I is described. Plasmid-less derivatives of these moderately resistant mutants ... -
Deletions in the r-determinant mer region of plasmid R100-1 selected by loss of mercury hypersensitivity
(1979)A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to ... -
Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1
(1979)A series of 23 transposon 801(Tn801)-induced mutations of plasmid R100-1 from mercuric salts resistance to sensitivity was studied. Although Tn801 transposed frequently into the mer region of the plasmid, fine structural ... -
Analysis of tetracycline resistance encoded by transposon Tn10: deletion mapping of tetracycline-sensitive point mutations and identification of two structural genes
(1983)Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. ... -
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100
(1983)The mercuric resistance (mer) genes of plasmid R100 were cloned into plasmid pBR322. A series of transposon Tn5 insertion mutations in the mer genes were isolated and mapped. The mutants were characterized phenotypically ... -
Expression of a cloned Staphylococcus aureus alpha-hemolysin determinant in Bacillus subtilis and Staphylococcus aureus
(1983)A DNA sequence encoding Staphylococcus aureus alpha-hemolysin, which had been previously cloned and mapped in Escherichia coli K-12, was introduced into Bacillus subtilis BD170 and several strains of S. aureus by using ... -
Some mercurial resistance plasmids from different incompatibility groups specify merR regulatory functions that both repress and induce the mer operon of plasmid R100
(1985)Transcription of the mer genes of plasmid R100 is regulated by the product of the merR gene. The merR gene negatively regulates its own expression and also controls the transcription of the merTCA operon both negatively ... -
Posttranscriptional regulation of the inducible nonenzymatic chloramphenicol resistance determinant of IncP plasmid R26
(1985)The inducible nonenzymatic chloramphenicol resistance (Cmr) determinant of the IncP plasmid R26 was cloned on a 1,900-base-pair restriction endonuclease HindIII fragment. Transposon Tn5 mutagenesis revealed that at least ... -
Identification of the merR gene of R100 by using mer-lac gene and operon fusions
(1985)Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were ... -
Genetic analysis of gentamicin resistance in methicillin- and gentamicin-resistant strains of Staphylococcus aureus isolated in Dublin hospitals.
(American Society for MIcrobiology, 1988)Methicillin- and gentamicin-resistant strains of Staphylococcus aureus isolated in Dublin hospitals have been classified into groups I, II, and III based on resistance to antimicrobial agents and plasmid profiles. Each ... -
Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor
(1992)Staphylococcus aureus Newman cells carry a surface receptor for fibrinogen called clumping factor. The bacteria also express coagulase, an extracellular protein that binds to prothrombin to form a complex with thrombinlike ... -
Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus
(1993)The effect of an agr mutation on expression of type 5 capsular polysaccharide (CP) by Staphylococcus aureus Newman was investigated in different complex and synthetic media. CP expression by the agr mutant was strongly ... -
The gamma-hemolysin locus of Staphylococcus aureus comprises three linked genes, two of which are identical to the genes for the F and S components of leukocidin
(1993)The Staphylococcus aureus gamma-hemolysin comprises two polypeptides, whereas the gamma-hemolysin locus (hlg) contains three open reading frames. The hlgA and hlgB genes encode the gamma 1 and gamma 2 components, respectively. ... -
Recombination at the coagulase locus in Staphylococcus aureus: plasmid integration and amplification
(1993)The integrating plasmid pCOA18, comprising pUC18 linked to a mutated coagulase (coa) gene from Staphylococcus aureus, and constructed by substituting coa sequences with a tetracycline (Tc)-resistance marker (delta coa::Tcr), ... -
Use of adhesion-defective mutants of Staphylococcus aureus to define the role of specific plasma proteins in promoting bacterial adhesion to canine arteriovenous shunts
(1995)We used an ex vivo canine arteriovenous shunt model, previously developed to study plasma protein adsorption and thrombogenesis on polymeric biomaterials, to define the role of host proteins in promoting adhesion of ... -
Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis.
(American Society for Microbiology, 1995)A deletion of the spoIIAC gene of Bacillus licheniformis was prepared in vitro by using the splicing-by-overlapextension technique. This gene was introduced into B. licheniformis on a temperature-sensitive plasmid, and ...
