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dc.contributor.advisorBradley, Louise
dc.contributor.authorMcGrath, Fionnuala
dc.date.accessioned2023-06-22T20:51:04Z
dc.date.available2023-06-22T20:51:04Z
dc.date.issued2023en
dc.date.submitted2023
dc.identifier.citationMcGrath, Fionnuala, Optical Characterisation of Metallic and Biological Nanoparticles, Trinity College Dublin, School of Physics, Physics, 2023en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/102983
dc.descriptionAPPROVEDen
dc.description.abstractNanotechnology is a rapidly growing technology with applications that affect many industries in the global economy. Two such industries are optoelectronics and healthcare. With the increased demand for smaller, more efficient, and more affordable device, there is also an increased need for smaller optoelectronic components. In this work, fabrication of metasurfaces using a solution-based nanoimprint lithography process is investigated. The properties of silver nanoparticle ink grating and planar samples, with and without a PMMA layers, are compared with thermally evaporated silver on a nanoimprinted PMMA layers. The grating pattern is successfully fabricated using nanoimprint lithography with the silver nanoparticle ink directly on a glass substrate and on a PMMA layer. The use of PMMA was found to adversely affect the structural, optical and electrical properties of the silver nanoparticle planar surfaces and gratings. Gratings nanoimprinted directly into silver nanoparticle ink on a glass substrate were found to have similar performance to gratings produced by thermal evaporation of silver on nanoimprinted PMMA and benefit from an easier fabrication process. The silver nanoparticle ink shows promise for the fabrication of patterned silver thin films and may be useful for upscaling and roll-to-roll processes. The application of nanotechnology in medicine, nanomedicine, is an area of technology where there have been significant breakthroughs. Nanobiomaterials (NBM) are studied extensively within nanomedicine with applications in drug delivery, contact tracing, deep-tissue imaging, and the detection and treatment of diseases. Pre-clinical assessment is an important step in the development of new NBMs to examine the toxicity of the NBMs. The current state-of-the-art to assess toxicity is to use two- dimensional (2D) cell cultures. These in vitro 2D assays lack the physiological process present in in vivo models. However, toxicity observed in in vivo animal models is not always observed in humans, and can make predicting possible interactions difficult. Three-dimensional (3D) in vitro models are currently being investigated as possible alternatives to 2D in vitro and in vivo animal models. These 3D models, or spheroids, are more biological relevant than their 2D predecessors, with some studies using them in pre-clinical investigations to examine dosing effects of nanoparticles. In this work, two NBMs, LipImage™815,a liposome loaded with a near infrared dye (IR780), and PACA(NR668), a polymeric shell loaded with far red dye (NR668), are investigated. Using confocal microscopy, a powerful fluorescence imaging modality, the translocation and penetration of the NBMs into the spheroid is assessed. The initial work is carried out over a 72 h period using one concentration for each NBM. Fluorescence intensity is observed decreasing at treatments times of up to 72 h, for both NBMs. However, the rate at which they penetrate to the core, and become homogenous differs for both NBMs. Fluorescence Lifetime Imaging Microscopy (FLIM) also showed that concentration, treatment time and translocation have no affect on the lifetime of the NBMs, suggesting the decrease in intensity observed at longer treatment times is due to degradation of the liposome or polymeric shell, and quenching of the encapsulated dyes.en
dc.language.isoenen
dc.publisherTrinity College Dublin. School of Physics. Discipline of Physicsen
dc.rightsYen
dc.titleOptical Characterisation of Metallic and Biological Nanoparticlesen
dc.typeThesisen
dc.type.supercollectionthesis_dissertationsen
dc.type.supercollectionrefereed_publicationsen
dc.type.qualificationlevelDoctoralen
dc.identifier.peoplefinderurlhttps://tcdlocalportal.tcd.ie/pls/EnterApex/f?p=800:71:0::::P71_USERNAME:MCGRATFIen
dc.identifier.rssinternalid256647en
dc.rights.ecaccessrightsopenAccess


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