Electrospun Fibres of Chitosan/PVP for the effective chemotherapeutic drug delivery of 5-Fluorouracil
Citation:
James Grant, Suresh Pillai, Sarah Hehir, Tatiana S. Perova, S. Hinder, Marion McCafee, Ailish Breen, Electrospun Fibres of Chitosan/PVP for the effective chemotherapeutic drug delivery of 5-Fluorouracil, Chemosensors, 2021, 9, 70Download Item:
Abstract:
Electrospun nanofibrous mats consisting of chitosan (CS) and polyvinylpyrrolidone (PVP)
were constructed. Tuning of solution and process parameters was performed and resulted in an
electrospun system containing a 6:4 ratio of PVP:CS. This is a significant increase in the proportion
of spun CS on the previously reported highest ratio PVP:CS blend. SEM analysis showed that the
nanofibrous mats with 4 wt% CS/6 wt% PVP (sample E) comprised homogenous, uniform fibres
with an average diameter of 0.569 μm. XPS analysis showed that the surface of the samples consisted of PVP. Raman and FTIR analysis revealed intermolecular interactions (via H-bonding) between PVP and CS. In FTIR spectra, the contribution of chitosan to CS/PVP complexes was shown by the downshift of the C=O band and by the linear increase in intensity of C-O stretching in CS. XPS
analysis showed a smaller shift at the binding energy 531 eV, which relates to the amide of the
acetylated functional groups. The obtained results demonstrate a sensitivity of Raman and FTIR tests
to the presence of chitosan in PVP:CS blend. The chemotherapy drug 5-Fu was incorporated into the
constructs and cell viability studies were performed. WST-8 viability assay showed that exposure
of A549 human alveolar basal epithelial cells to 10 mg/mL 5-Fu loaded fibres was most effective at
killing cells over 24 h. On the other hand, the constructs with loading of 1 mg/mL of drug were not
efficient at killing A549 human alveolar basal epithelial cells. This study showed that CS/PVP/5-Fu
constructs have potential in chemotherapeutic drug delivery systems.
Author's Homepage:
http://people.tcd.ie/perovat
Author: Perova, Tatiana
Type of material:
Journal ArticleSeries/Report no:
Chemosensors;9;
4;
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Full text availableKeywords:
5-Fluorouracil, Cell viability, FTIR, Raman, XPS, Nanofibers, Electrospinning, PVP, ChitosanSubject (TCD):
Nanoscience & Materials , BiomaterialsDOI:
https://doi.org/10.3390/chemosensors9040070Metadata
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