Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering
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2019Author:
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Rathan, S., Dejob, L., Schipani, R., Haffner, B., Möbius, M.E. & Kelly, D.J., Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering, 2019, Advanced Healthcare Materials, 8, 7Download Item:
Rathan et al 2019 Preprint.pdf (Accepted for publication (author's copy) - Peer Reviewed) 1.473Mb
Abstract:
Focal articular cartilage (AC) defects, if left untreated, can lead to debilitating diseases such as
osteoarthritis. While several tissue engineering strategies have been developed to promote cartilage
regeneration, it is still challenging to generate functional AC capable of sustaining high load-bearing
environments. We developed a new class of cartilage extracellular matrix (cECM)-functionalized
alginate bioink for the bioprinting of cartilaginous tissues. The bioinks were 3D-printable, supported
mesenchymal stem cell (MSC) viability post-printing and robust chondrogenesis in vitro, with the highest
levels of COLLII and ACAN expression observed in bioinks containing the highest concentration of
cECM. Enhanced chondrogenesis in cECM-functionalized bioinks was also associated with progression
along an endochondral-like pathway, as evident by increases in RUNX2 expression and calcium
deposition in vitro. The bioinks loaded with MSCs and TGF-β3 were also found capable of supporting
robust chondrogenesis, opening the possibility of using such bioinks for direct ‘print-and-implant’
cartilage repair strategies. Finally, we demonstrated that networks of 3D-printed polycaprolactone fibers
with compressive modulus comparable to native AC could be used to mechanically reinforce these
bioinks, with no loss in cell viability. It is envisioned that combinations of such biomaterials can be used
in multiple-tool biofabrication strategies for the bioprinting of biomimetic cartilaginous implants.
URI:
https://onlinelibrary.wiley.com/doi/full/10.1002/adhm.201801501http://hdl.handle.net/2262/91275
Sponsor
Grant Number
Science Foundation Ireland
12/IA/1554
Author's Homepage:
http://people.tcd.ie/kellyd9http://people.tcd.ie/mobiusm
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PUBLISHED
Author: Moebius, Matthias; Kelly, Daniel; Rathan, Swetha; Dejob, Léa; Schipani, Rossana; Haffner, Benjamin
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Journal ArticleURI:
https://onlinelibrary.wiley.com/doi/full/10.1002/adhm.201801501http://hdl.handle.net/2262/91275
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Advanced Healthcare Materials;Availability:
Full text availableKeywords:
3D-bioprinting, Articular cartilage tissue engineering, Extracellular matrix bioinks, Growth factor, PolycaprolactoneDOI:
http://dx.doi.org/10.1002/adhm.201801501ISSN:
21922659 21922640Licences: