Putting oesophageal cancer on the RACK A study of the RACK1 scaffolding protein in oesophageal adenocarcinoma
Citation:
Deirdre Duff, 'Putting oesophageal cancer on the RACK A study of the RACK1 scaffolding protein in oesophageal adenocarcinoma', Graduate Students’ Union of the University of Dublin, Trinity College, Journal of Postgraduate Research;, 2015Download Item:
JPR_2015_set_digital_duff.pdf (PDF) 1.473Mb
Abstract:
Purpose
There is a need to identify new therapeutic targets for oesophageal adenocarcinoma.
Deoxycholic acid is a component of gastro-oesophageal refluxate and is believed to
promote oesophageal adenocarcinoma. RACK1 is an intracellular scaffolding protein
whose expression is altered in many cancers. The role of RACK1 in oesophageal
adenocarcinoma has not been investigated. The purpose of this study is to examine
the expression of RACK1 in oesophageal adenocarcinoma and to assess its potential
as a contributor to this disease. This study also aims to investigate if deoxycholic acid
modulates RACK1 in oesophageal adenocarcinoma cells.
Methods
Normal oesophageal epithelial cell lines, Barrett’s metaplastic and dysplastic cell
lines and oesophageal adenocarcinoma cell lines were used to construct a model of
oesophageal adenocarcinoma disease progression.
RACK1 expression levels were assessed by western blotting.
RACK1 subcellular localisation was examined by immunofluorescent confocal microscopy.
Results
RACK1 protein levels generally increase with increasing disease progression across
a panel of cell lines representing the multistep sequence from normal oesophagus to
oesophageal adenocarcinoma.
Deoxycholic acid downregulates RACK1 protein levels in SKGT-4 (31% reduction;
P=0.01) and FLO-1 (26% reduction; P=0.03) oesophageal adenocarcinoma cells.
RACK1 has a diffuse, cytoplasmic localisation in SKGT-4 and FLO-1 cells and is most
concentrated in the perinuclear area. This pattern of RACK1 subcellular localisation
is not altered by deoxycholic acid
Conclusion
RACK1 may be upregulated during the development of oesophageal adenocarcinoma.
Deoxycholic acid can modulate RACK1 protein levels in oesophageal adenocarcinoma
cells. RACK1 may contribute to the progression of oesophageal adenocarcinoma and
further investigation into its role in this disease is warranted.
Author: Duff, Deirdre
Publisher:
Graduate Students’ Union of the University of Dublin, Trinity CollegeType of material:
Journal ArticleCollections:
Series/Report no:
Journal of Postgraduate Research;Availability:
Full text availableKeywords:
deoxycholic acid, RACK1, Oesophageal adenocarcinomaISSN:
2009-4787Licences: