GENOTYPING Candida albicans FROM CANDIDA LEUKOPLAKIA AND NON-CANDIDA LEUKOPLAKIA SHOWS NO ENRICHMENT OF MULTILOCUS SEQUENCE TYPING CLADES BUT ENRICHMENT OF ABC GENOTYPE C IN CANDIDA LEUKOPLAKIA
File Type:
PDFItem Type:
Journal ArticleDate:
2013Access:
OpenAccessCitation:
ABDULRAHIM MH, McMANUS BA, FLINT SR, COLEMAN DC, GENOTYPING Candida albicans FROM CANDIDA LEUKOPLAKIA AND NON-CANDIDA LEUKOPLAKIA SHOWS NO ENRICHMENT OF MULTILOCUS SEQUENCE TYPING CLADES BUT ENRICHMENT OF ABC GENOTYPE C IN CANDIDA LEUKOPLAKIA, PLOS ONE, 8, 9, 2013, 1-11:e73738Abstract:
Oral leukoplakias are histopathologically-diagnosed as Candida leukoplakia or non-Candida leukoplakia by the presence or absence of hyphae in the superficial epithelium. Candida leukoplakia lesions have significantly increased malignant potential. Candida albicans is the most prevalent fungal species associated with oral leukoplakia and may contribute to malignant transformation of Candida leukoplakia. To date, no detailed population analysis of C. albicans isolates from oral leukoplakia patients has been undertaken. This study investigated whether specific C. albicans genotypes were associated with Candida leukoplakia and non-Candida leukoplakia in a cohort of Irish patients.
Patients with histopathologically-defined Candida leukoplakia (n=31) or non-Candida leukoplakia (n=47) were screened for Candida species by culture of oral rinse and lesional swab samples. Selected C. albicans isolates from Candida leukoplakia patients (n=25), non-Candida leukoplakia patients (n=19) and oral carriage isolates from age and sex matched healthy subjects without leukoplakia (n=34) were subjected to multilocus sequence typing (MLST) and ABC genotyping.
MLST revealed that the clade distribution of C. albicans from both Candida leukoplakia and non-Candida leukoplakia lesions overlapped with the corresponding clade distributions of oral carriage isolates and global reference isolates from the MLST database indicating no enrichment of leukoplakia-associated clones.
Oral leukoplakia isolates were significantly enriched with ABC genotype C (12/44, 27.3%), particularly Candida leukoplakia isolates (9/25, 36%), relative to oral carriage isolates (3/34, 8.8%). Genotype C oral leukoplakia isolates were distributed in MLST clades 1,3,4,5,8,9 and 15, whereas genotype C oral carriage isolates were distributed in MLST clades 4 and 11.
Author's Homepage:
http://people.tcd.ie/dcolemanhttp://people.tcd.ie/bmcmanu
Description:
PUBLISHEDPublished: September 18th 2013
Author: MCMANUS, BRENDA; COLEMAN, DAVID
Type of material:
Journal ArticleCollections
Series/Report no:
PLOS ONE8
9
Availability:
Full text availableKeywords:
Candida leukoplakia, MLST, ABC genotyping, Candida albicansSubject (TCD):
Immunology, Inflammation & InfectionDOI:
http://dx.doi.org/10.1371/journal.pone.0073738Metadata
Show full item recordLicences:
Related items
Showing items related by title, author, creator and subject.
-
Characterisation of Candida albicans populations associated with chronic hyperplastic candidosis (Candida leukoplakia) and non-Candida leukoplakia lesions in patients presenting with oral leukoplakic lesions in Ireland
Abdulrahim, Mohammed H. (Trinity College (Dublin, Ireland). School of Dental Science, 2011)Oral squamous cell carcinoma is a significant global health problem, affecting about a half of a million people worldwide annually. Oral leukoplakic lesions have been reported to have significantly increased potential for ... -
Comparative analysis of the filamentous growth regulators EED1 in Candida albicans and MDP1 in Candida dubliniensis
O'Connor, Leanne (Trinity College (Dublin, Ireland). School of Dental Science, 2009)Candida albicans, the major fungal pathogen in humans is closely related to Candida dubliniensis. C. dubliniensis is less virulent than C. albicans in all models of infection. Comparative analysis of the C. albicans and ... -
Comparative growth analysis of Candida dubliniensis and Candida albicans in vitro and in selected infection models
Stokes, Cheryl (Trinity College (Dublin, Ireland). School of Dental Science, 2006)Candida dubliniensis is the species most closely related to C. albicans and is primarily associated with colonisation and oral candidiasis in Human Immunodeficiency Virus (HlV)-infected individuals and AIDS patients. Despite ...