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dc.contributor.authorSHEILS, ORLA
dc.contributor.authorO'LEARY, JOHN JAMES
dc.contributor.authorO'TOOLE, SHARON
dc.contributor.authorMARTIN, CARA
dc.date.accessioned2013-11-15T12:57:47Z
dc.date.available2013-11-15T12:57:47Z
dc.date.issued2008
dc.date.submitted2008en
dc.identifier.citationLaios, A., O'Toole, S., Flavin, R., Martin, C., Kelly, L., Ring, M., Finn, S.P., (...), O'Leary, J., Potential role of miR-9 and miR-223 in recurrent ovarian cancer , Molecular Cancer, 7, 2008, art. no. 35en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/67627
dc.descriptionPUBLISHEDen
dc.description.abstractBackground: MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression by binding to target mRNAs. miRNAs have not been comprehensively studied in recurrent ovarian cancer, yet an incurable disease. Results: Using real-time RT-PCR, we obtained distinct miRNA expression profiles between primary and recurrent serous papillary ovarian adenocarcinomas (n = 6) in a subset of samples previously used in a transcriptome approach. Expression levels of top dysregulated miRNA genes, miR-223 and miR-9, were examined using TaqMan PCR in independent cohorts of fresh frozen (n = 18) and FFPE serous ovarian tumours (n = 22). Concordance was observed on TaqMan analysis for miR-223 and miR-9 between the training cohort and the independent test cohorts. Target prediction analysis for the above miRNA "recurrent metastatic signature" identified genes previously validated in our transcriptome study. Common biological pathways well characterised in ovarian cancer were shared by miR-9 and miR-223 lists of predicted target genes. We provide strong evidence that miR-9 acts as a putative tumour suppressor gene in recurrent ovarian cancer. Components of the miRNA processing machinery, such as Dicer and Drosha are not responsible for miRNA deregulation in recurrent ovarian cancer, as deluded by TaqMan and immunohistochemistry. Conclusion: We propose a miRNA model for the molecular pathogenesis of recurrent ovarian cancer. Some of the differentially deregulated miRNAs identified correlate with our previous transcriptome findings. Based on integrated transcriptome and miRNA analysis, miR-9 and miR-223 can be of potential importance as biomarkers in recurrent ovarian cancer.en
dc.format.extentart. no. 35en
dc.language.isoenen
dc.relation.ispartofseriesMolecular Cancer;
dc.relation.ispartofseries7;
dc.rightsYen
dc.subjectMICRORNA EXPRESSIONen
dc.subjectGENEen
dc.subjectTRANSCRIPTIONen
dc.titlePotential role of miR-9 and miR-223 in recurrent ovarian canceren
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/shotoole
dc.identifier.peoplefinderurlhttp://people.tcd.ie/osheils
dc.identifier.peoplefinderurlhttp://people.tcd.ie/olearyjj
dc.identifier.peoplefinderurlhttp://people.tcd.ie/cmartin3
dc.identifier.rssinternalid59239
dc.rights.ecaccessrightsOpenAccess


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