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dc.contributor.authorO'SULLIVAN, JACINTHAen
dc.date.accessioned2012-06-27T11:28:06Z
dc.date.available2012-06-27T11:28:06Z
dc.date.issued2012en
dc.date.submitted2012en
dc.identifier.citationLeonard C Harty, Monika Biniecka, Jacintha O'Sullivan, Edward Fox, Kevin Mulhall, Douglas J Veale, Ursula Fearon, Mitochondrial mutagenesis correlates with the local inflammatory environment in arthritis, Annals of the Rheumatic Diseases: The EULAR Journal, 71, 4, 2012, 582-588en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/64035
dc.descriptionPUBLISHEDen
dc.description.abstractBackground: To examine the association between mitochondrial mutagenesis and the proinflammatory microenvironment in patients with inflammatory arthritis. Methods: Fifty patients with inflammatory arthritis underwent arthroscopy and synovial tissue biopsies, synovial fluid and clinical assessment were obtained. Fifteen patients pre/post-TNFi therapy were also recruited. Normal synovial biopsies were obtained from 10 subjects undergoing interventional arthroscopy. Macroscopic synovitis/vascularity was measured by visual analogue scale. Cell-specific markers CD3 (T cells) and CD68 (macrophages) were quantified by immunohistology. TNF?, IL-6, IFN? and IL-1? were measured in synovial fluids by MSD multiplex assays. Synovial tissue mitochondrial mutagenesis was quantified using a mitochondrial random mutation capture assay (RMCA). The direct effect of TNF? on oxidative stress and mitochondrial function was assessed in primary cultures of rheumatoid arthritis synovial fibroblast cells (RASFCs). Mitochondrial mutagenesis, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and mitochondrial mass (MM) were quantified using the RMCA and specific cell fluorescent probes. Results: A significant increase in mtDNA mutation frequency was demonstrated in inflamed synovial tissue compared with control (p<0.05), an effect that was independent of age. mtDNA mutations positively correlated with macroscopic synovitis (r=0.52, p<0.016), vascularity (r=0.54, p<0.01) and with synovial fluid cytokine levels of TNF? (r=0.74, p<0.024) and IFN? (r=0.72, p<0.039). mtDNA mutation frequency post-TNFi therapy was significantly lower in patients with a DAS<3.2 (p<0.05) and associated with clinical and microscopic measures of disease (p<0.05). In vitro TNF? significantly induced mtDNA mutations, ROS, MM and MMP in RASFCs (all p<0.05). Conclusion: High mitochondrial mutations are strongly associated with synovial inflammation showing a direct link between mitochondrial mutations and key proinflammatory pathways.en
dc.description.sponsorshipThis study was funded by the Centocor Newman Fellowship, the Health Research Board of Ireland and EU FP6 AutoCure.en
dc.format.extent582-588en
dc.language.isoenen
dc.relation.ispartofseriesAnnals of the Rheumatic Diseases: The EULAR Journalen
dc.relation.ispartofseries71en
dc.relation.ispartofseries4en
dc.rightsYen
dc.subjectRheumatologyen
dc.subjectinflammatory arthritisen
dc.subjectmitochondrial mutagenesisen
dc.subjectarthroscopyen
dc.titleMitochondrial mutagenesis correlates with the local inflammatory environment in arthritisen
dc.typeJournal Articleen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorHealth Research Board (HRB)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/osullij4en
dc.identifier.rssinternalid76081en
dc.identifier.doihttp://dx.doi.org/10.1136/annrheumdis-2011-200245en
dc.subject.TCDThemeNeuroscienceen
dc.identifier.rssurihttp://dx.doi.org/10.1136/annrheumdis-2011-200245en


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