dc.contributor.author | Fleming, Garry | en |
dc.contributor.author | Moran, Gary | en |
dc.date.accessioned | 2012-01-12T15:04:08Z | |
dc.date.available | 2012-01-12T15:04:08Z | |
dc.date.issued | 2012 | en |
dc.date.submitted | 2012 | en |
dc.identifier.citation | McGinley, E.L., Moran, G.P., Fleming, G.J.P., Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model, Acta Biomaterialia, 8, 1, 2012, 432 - 438 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/61635 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV nickel-induced hypersensitivity. We hypothesized the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into mechanisms of nickel-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto Alloderm? and maintained until full-thickness was achieved prior to Ni-Cr and cobalt-chromium (Co-Cr) alloy-disc exposure (2-72 h). Biocompatibility assessment involved histological analyses with cell viability measurements, oxidative stress responses, inflammatory cytokine expression and cellular toxicity analyses. Inductively coupled plasma mass spectrometry (ICP-MS) analysis determined elemental ion release levels. We detected adverse morphology with significant reductions in cell viability, significant increases in oxidative stress, inflammatory cytokine expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models compared with untreated oral mucosal models and adverse effects were increased for the Ni-Cr alloy that leached the most nickel. Co-Cr demonstrated significantly enhanced biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-derived full-thickness oral mucosal model was discriminatory between dental alloys and mechanistically provided insights into Ni-induced toxicity, highlighting potential clinical relevance. | en |
dc.format.extent | 432 | en |
dc.format.extent | 438 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Acta Biomaterialia | en |
dc.relation.ispartofseries | 8 | en |
dc.relation.ispartofseries | 1 | en |
dc.rights | Y | en |
dc.subject | Dentistry | en |
dc.subject | Nickel-chromium (Ni-Cr) | en |
dc.subject | alloys | en |
dc.subject | Biocompatibility assessment | en |
dc.title | Base-metal dental casting alloy biocompatibility assessment using a human-derived 3D oral mucosal model | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/flemingg | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/gmoran | en |
dc.identifier.rssinternalid | 74712 | en |
dc.subject.TCDTheme | Nanoscience & Materials | en |
dc.subject.TCDTheme | Next Generation Medical Devices | en |
dc.identifier.rssuri | http://dx.doi.org/10.1016/j.actbio.2011.08.017 | en |